Investigators at Laval University Report Findings in Bacillus anthracis (Portable bead-based fluorescence detection system for multiplex nucleic acid testing: a case study with Bacillus anthracis)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Gram-Positive Bacteria are presented in a new report. According to news reporting out of Quebec City, Canada, by NewsRx editors, research stated, "This paper describes the design, functioning and use of a portable detection platform for multiplex nucleic acid testing. The system features a bead-supported DNA hybridization assay performed inside a microfluidic cartridge."
Our news journalists obtained a quote from the research from Laval University, "Polystyrene particles modified with DNA capture probes are confined in the detection area and exposed to a solution of fluorescently labeled target DNA strands. The cartridge, fabricated from inexpensive thermoplastic polymers, allows for conducting up to eight assays in parallel. The detection instrument is equipped with a pneumatic module and a manifold lid serving as an interface to mediate fluid displacement on the cartridge. The fluorescence signal deriving from each assay is recorded by a semi-confocal fluorescence reader embedded in the detection platform. The compact design of the instrument and its level of integration make it possible to obtain an analytical result in less than 15 min, while only few manual steps need to be performed in between. A proof-of-concept demonstration involving Cy3-labeled, PCR-amplified genomic DNA confirms the ability to detect Bacillus anthracis in a multiplexed single-assay format using lef and capC genes."
According to the news editors, the research concluded: "Limits of quantification are on the order of 1 x 10(9) copies/mu L for lef targets."
For more information on this research see: Portable bead-based fluorescence detection system for multiplex nucleic acid testing: a case study with Bacillus anthracis. Microfluidics and Nanofluidics, 2014;16(6):1075-1087. Microfluidics and Nanofluidics can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Microfluidics and Nanofluidics - www.springerlink.com/content/1613-4982/)
Our news journalists report that additional information may be obtained by contacting J.F. Gravel, Laval University, Center Rech CHUQ, Center Rech Infectiol, Quebec City, PQ G1V 4G2, Canada. Additional authors for this research include M. Geissler, S. Chapdelaine, K. Boissinot, B. Voisin, I. Charlebois, H.P. Poirier-Richard, A. Gregoire, M. Boissinot, M.G. Bergeron, T. Veres and D. Boudreau (see also Gram-Positive Bacteria).
Keywords for this news article include: Canada, Quebec City, Bacillaceae, Bacillus anthracis, Gram-Positive Rods, Gram-Positive Bacteria, North and Central America, Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods
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