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Investigators at Center for Advanced Biotechnology and Medicine Release New Data on Peptides and Proteins (Extending the mannose 6-phosphate...

August 12, 2014



Investigators at Center for Advanced Biotechnology and Medicine Release New Data on Peptides and Proteins (Extending the mannose 6-phosphate glycoproteome by high resolution/accuracy mass spectrometry analysis of control and acid phosphatase ...)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Peptides and Proteins have been published. According to news reporting from Piscataway, New Jersey, by NewsRx journalists, research stated, "In mammals, most newly synthesized lumenal lysosomal proteins are delivered to the lysosome by the mannose 6-phosphate (Man6P) targeting pathway. Man6P -containing proteins can be affinity-purified and characterized using proteomic approaches, and such studies have led to the discovery of new lysosomal proteins and associated human disease genes."

The news correspondents obtained a quote from the research from Center for Advanced Biotechnology and Medicine, "One limitation to this approach is that in most cell types the Man6P modification is rapidly removed by acid phosphatase 5 (ACP5) after proteins are targeted to the lysosome, and thus, some lysosomal proteins may escape detection. In this study, we have extended the analysis of the lysosomal proteome using high resolution/accuracy mass spectrometry to identify and quantify proteins in a combined analysis of control and ACP5-deficient mice. To identify Man6P glycoproteins with limited tissue distribution, we analyzed multiple tissues and used statistical approaches to identify proteins that are purified with high specificity. In addition to 68 known Man6P glycoproteins, 165 other murine proteins were identified that may contain Man6P and may thus represent novel lysosomal residents."

According to the news reporters, the research concluded: "For four of these lysosomal candidates, (lactoperoxidase, phospholipase D family member 3, ribonuclease 6, and serum amyloid P component), we demonstrate lysosomal residence based on the colocalization of fluorescent fusion proteins with a lysosomal marker."

For more information on this research see: Extending the mannose 6-phosphate glycoproteome by high resolution/accuracy mass spectrometry analysis of control and acid phosphatase 5-deficient mice. Molecular and Cellular Proteomics, 2013;12(7):1806-17. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Molecular and Cellular Proteomics - www.mcponline.org/)

Our news journalists report that additional information may be obtained by contacting D.E. Sleat, Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, United States. Additional authors for this research include P. Sun, J.A. Wiseman, L. Huang, M. El-Banna, H. Zheng, D.F. Moore and P. Lobel (see also Peptides and Proteins).

Keywords for this news article include: Anions, Piscataway, New Jersey, Phosphates, Amino Acids, United States, Glycoproteins, Glycoconjugates, Phosphoric Acids, Peptides and Proteins, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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