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Findings on Molecular Biology Detailed by Investigators at Jilin University (Expression, Purification, and Characterization of Cu/ZnSOD from Panax...

August 12, 2014



Findings on Molecular Biology Detailed by Investigators at Jilin University (Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng)

By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Life Science Research have been presented. According to news reporting originating in Changchun, People's Republic of China, by NewsRx journalists, research stated, "Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220."

The news reporters obtained a quote from the research from Jilin University, "Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD in the bacterial broth, with a protein content of 965 mg/L and enzyme specific activity of 9389.96 U/mg."

According to the news reporters, the research concluded: "These results provide a foundation for future studies on the antioxidant mechanisms of ginseng and the development and application of ginseng Cu/ZnSOD."

For more information on this research see: Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng. Molecules, 2014;19(6):8112-8123. Molecules can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland. (Springer - www.springer.com; Molecules - www.springerlink.com/content/1420-3049/)

Our news correspondents report that additional information may be obtained by contacting D.Y. Ding, Jilin University, China Japan Union Hosp, Dept. of Geriatr, Changchun 130000, People's Republic of China. Additional authors for this research include S.C. Liu, K. Wang, L.H. Huang and J.S. Zhao (see also Life Science Research).

Keywords for this news article include: Asia, Changchun, Life Science Research, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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