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Findings from University of the West in the Area of Chromatography Reported (Comparison of peak shape in hydrophilic interaction chromatography using...

August 12, 2014



Findings from University of the West in the Area of Chromatography Reported (Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions)

By a News Reporter-Staff News Editor at Journal of Technology -- Data detailed on Chromatography have been presented. According to news reporting from Bristol, United Kingdom, by VerticalNews journalists, research stated, "The retention and peak shape of neutral, basic and acidic solutes was studied on hydrophilic interaction chromatography (HILIC) stationary phases that showed both strong and weak ionic retention characteristics, using aqueous-acetonitrile mobile phases containing either formic acid (FA), ammonium formate (AF) or phosphoric acid (PA). The effect of organic solvent concentration on the results was also studied."

The news correspondents obtained a quote from the research from the University of the West, "Peak shape was good for neutrals under most mobile phase conditions. However, peak shapes for ionised solutes, particularly for basic compounds, were considerably worse in FA than AF. Even neutral compounds showed deterioration in performance with FA when the mobile phase water concentration was reduced. The poor performance in FA cannot be entirely attributed to the negative impact of ionic retention on ionised silanols on the underlying silica base materials, as results using PA at lower pH (where their ionisation is suppressed) were inferior to those in AF."

According to the news reporters, the research concluded: "Besides the moderating influence of the salt cation on ionic retention, it is likely that salt buffers improve peak shape due to the increased ionic strength of the mobile phase and its impact on the formation of the water layer on the column surface."

For more information on this research see: Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions. Journal of Chromatography A, 2014;1347():39-48. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)

Our news journalists report that additional information may be obtained by contacting J.C. Heaton, Centre for Research in Biosciences, University of the West of Engl andFrenchay, Bristol BS16 1QY, UK. Additional authors for this research include J.J. Russell, T. Underwood, R. Boughtflower and D.V McCalley.

Keywords for this news article include: Europe, Bristol, United Kingdom, Chromatography.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Journal of Technology


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