Findings from Jiangnan University Yields New Data on Staphylococcus aureus (A sensitive gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureus)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Gram-Positive Bacteria. According to news reporting originating in Wuxi, People's Republic of China, by NewsRx journalists, research stated, "In this study, a gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureus (S. aureus) using tyramine signal amplification (TSA) technology has been developed. First, the biotinylated aptamer specific for S. aureus was immobilized on the surface of the wells of the microtiter plate via biotin-avidin binding."
The news reporters obtained a quote from the research from Jiangnan University, "Then, the target bacteria (S. aureus), biotinylated-aptamer-streptavidin-HRP conjugates, biotinylated tyramine, hydrogen peroxide and avidin-catalase were successively introduced into the wells of the microtiter plate. After that, the existing catalase consumed the hydrogen peroxide. Finally, the freshly prepared gold (III) chloride trihydrate was added, the color of the reaction production would be changed and the absorbance at 550 nm could be measured with a plate reader. Under optimized conditions, there was a linear relationship between the absorbance at 550 nm and the concentration of S. aureus over the range from 10 to 10(6) cfu mL(-1) (with an R-2 of 0.9947)."
According to the news reporters, the research concluded: "The limit of the developed method was determined to be 9 cfu mL(-1)."
For more information on this research see: A sensitive gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureus. Talanta, 2014;127():163-168. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news correspondents report that additional information may be obtained by contacting J.L. Yuan, Jiangnan Univ, Sch Food Sci & Technol, Synerget Innovat Center Food Safety & Nutr, State Key Lab Food Sci & Technol, Wuxi 214122, People's Republic of China. Additional authors for this research include S.J. Wu, N. Duan, X.Y. Ma, Y. Xia, J. Chen, Z.S. Ding and Z.P. Wang (see also Gram-Positive Bacteria).
Keywords for this news article include: Wuxi, Asia, Catalase, Chemicals, Chemistry, Bacillales, Enzymology, Nanotechnology, Hydrogen Peroxide, Staphylococcaceae, Gold Nanoparticles, Gram-Positive Cocci, Emerging Technologies, Staphylococcus aureus, Gram-Positive Bacteria, People's Republic of China, Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods
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