Findings from G.E. Zemtsova and Colleagues Update Understanding of Medical Entomology [Multiplex qPCR Assay for Identification and Differentiation of Amblyomma americanum, Amblyomma cajennense, and Amblyomma maculatum (Ixodida: Ixodidae) Tick ...]
By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Health and Medicine. According to news reporting originating in Ann Arbor, Michigan, by NewsRx journalists, research stated, "Many ticks of the genus Amblyomma are vectors of human pathogens, and the correct species identification is medically and epidemiologically important. Morphological identification is time-consuming and requires a high level of expertise."
The news reporters obtained a quote from the research, "Identification of engorged, immature, or damaged ticks and the differentiation of closely related species remain problematic. Here, we report the development of a real-time TaqMan assay for the genomic identification and differentiation of Amblyomma americanum (L.), Amblyomma cajennense (F.), and Amblyomma maculatum (Koch), which are human-biting species found in the eastern United States. New species-specific sets of oligonucleotides for the multiplex reaction that detect and differentiate the ITS2 genomic regions of three target species were designed using Visual OMP; the previously published A. americanum oligonucleotide set was also incorporated into our assay. Specificity and sensitivity tests for two multiplex master mixes using different A. americanum sets were performed using individual and pooled samples of adult, nymphal, and larval ticks, and optimization procedures were applied. The multiplex assay successfully differentiates between genomes of three target species and does not cross-react with DNAs of ticks from other genera. Rare cases of nonspecific amplification occurred with DNAs of A. imitator and Amblyomma triste Koch misidentified as A. americanum and A. maculatum, respectively. However, this cross-reaction does not diminish the usefulness of the developed assay east of the 95th meridian, where neither A. imitator nor A. triste are found."
According to the news reporters, the research concluded: "Two master mixes incorporating the previously published or newly developed A. americanum sets are being recommended for identification of individual ticks or pooled samples, respectively."
For more information on this research see: Multiplex qPCR Assay for Identification and Differentiation of Amblyomma americanum, Amblyomma cajennense, and Amblyomma maculatum (Ixodida: Ixodidae) Tick Species in the Eastern United States. Journal of Medical Entomology, 2014;51(4):795-803. Journal of Medical Entomology can be contacted at: Entomological Soc Amer, 3 Park Place, Ste 307, Annapolis, MD 21401-3722, USA (see also Health and Medicine).
Our news correspondents report that additional information may be obtained by contacting G.E. Zemtsova, DNA Software Inc, Ann Arbor, MI 48104, United States. Additional authors for this research include N.E. Watkins and M.L. Levin.
Keywords for this news article include: Michigan, Ann Arbor, United States, Health and Medicine, North and Central America
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC