Findings from East China University of Science and Technology Provides New Data on Enzyme Research (Real-time assessment of the metabolic profile of living cells with genetically encoded NADH sensors)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Life Science Research is now available. According to news reporting from Shanghai, People's Republic of China, by NewsRx journalists, research stated, "Redox metabolism plays a critical role in multiple pathophysiological settings, including oncogenesis and tumor progression. Until recently, however, our knowledge of key redox processes in living systems was limited by the lack of an adequate methodology to monitor redox potential."
The news correspondents obtained a quote from the research from the East China University of Science and Technology, "Nicotinamide adenine dinucleotide, in its reduced (NADH) and oxidized (NAD(+)) forms, is perhaps the most important small molecule in the redox metabolism of mammalian cells. We have previously developed a series of genetically encoded fluorescent sensors allowing for the quantification of intracellular NADH. Here, we present experimental components and considerations that are required to perform a standardized quantification of intracellular NADH based on these probes. Moreover, we present the initial calibration experiments necessary to obtain reliable data from this approach, we detail a protocol to measure intracellular NADH levels in steady-state kinetic experiments, and we provide consideration on the processing of data."
According to the news reporters, the research concluded: "Among various applications, this technique is suitable for the study of redox alterations in malignant cells."
For more information on this research see: Real-time assessment of the metabolic profile of living cells with genetically encoded NADH sensors. Methods In Enzymology, 2014;542():349-67 (see also Life Science Research).
Our news journalists report that additional information may be obtained by contacting Y. Zhao, Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, People's Republic of China. Additional authors for this research include Y. Yang and J. Loscalzo.
Keywords for this news article include: Asia, Shanghai, Life Science Research, People's Republic of China.
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