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Reports Outline Infection Research Findings from P.M. Rath and Colleagues (Value of multiplex PCR using cerebrospinal fluid for the diagnosis of...

September 5, 2014



Reports Outline Infection Research Findings from P.M. Rath and Colleagues (Value of multiplex PCR using cerebrospinal fluid for the diagnosis of ventriculostomy-related meningitis in neurosurgery patients)

By a News Reporter-Staff News Editor at Health & Medicine Week -- New research on Health and Medicine is the subject of a report. According to news reporting originating in Hannover, Germany, by NewsRx journalists, research stated, "This prospective observational cohort study assessed the use of a multiplex real-time polymerase chain reaction (PCR) assay alone and in conjunction with biomarkers for the diagnosis of ventriculostomy-related meningitis in neurosurgery intensive care unit (ICU) patients with external ventricular drainage (EVD). Concentrations of intrathecal biomarkers, including lactate and interleukin 6 (IL-6), were measured, and cerebrospinal fluid (CSF) was examined microbiologically by blood culture BACTEC bottles in 62 CSF samples from 41 patients with EVD."

The news reporters obtained a quote from the research, "A portion of each sample was also tested with a commercially available PCR assay that simultaneously detects 25 species of bacteria and fungi [SeptiFast (SF)]. Receiver operating characteristic curve analysis was used to compare biomarker concentrations with SF and culture results. Seventeen (27 %) samples tested positive and 40 (65 %) tested negative for pathogens by both culture and SF. One pathogen was detected only by SF. Four samples tested positive by culture but negative by SF; in 3 of these, the isolates were considered to be contaminants. In comparison to CSF culture SF showed a larger area under the curve for IL-6 (0.90; 95 % CI 0.83-0.98) versus 0.70 (95 % CI 0.46-0.80) and for lactate (0.77; 95 % CI 0.63-0.93) versus 0.65 (95 % CI 0.50-0.80)."

According to the news reporters, the research concluded: "In 94 % (17/18) of positive SF samples the results were obtained on the same day whereas the overall mean of the time-to-positivity of BACTEC bottles was 21.6 h. The diagnosis of EVD-related ventriculo-meningitis in neurosurgical ICU patients can be established in a rapid manner using a multiplex PCR assay on CSF samples in combination with intrathecal biomarkers."

For more information on this research see: Value of multiplex PCR using cerebrospinal fluid for the diagnosis of ventriculostomy-related meningitis in neurosurgery patients. Infection, 2014;42(4):621-627. Infection can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Infection - www.springerlink.com/content/0300-8126/)

Our news correspondents report that additional information may be obtained by contacting P.M. Rath, TWINCORE Center Expt & Clin Infect Res, Div Expt Virol, Hannover, Germany. Additional authors for this research include B. Schoch, M. Adamzik, E. Steinmann, J. Buer and J. Steinmann (see also Health and Medicine).

Keywords for this news article include: Europe, Germany, Hannover, Health and Medicine

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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