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New Findings in Life Science Research Described from Max-Planck-Institute (Flux profiling of photosynthetic carbon metabolism in intact plants)

September 2, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting originating from Potsdam, Germany, by NewsRx correspondents, research stated, "Flux analysis has been carried out in plants for decades, but technical innovations are now enabling it to be carried out in photosynthetic tissues in a more precise fashion with respect to the number of metabolites measured. Here we describe a protocol, using gas chromatography (GC)- and liquid chromatography (LC)-mass spectrometry (MS), to resolve intracellular fluxes of the central carbon metabolism in illuminated intact Arabidopsis thaliana rosettes using the time course of the unlabeled fractions in 40 major constituents of the metabolome after switching to (CO2)-C-13."

Our news editors obtained a quote from the research from Max-Planck-Institute, "We additionally simplify modeling assumptions, specifically to cope with the presence of multiple cellular compartments. We summarize all steps in this 8-10-week-long process, including setting up the chamber; harvesting; liquid extraction and subsequent handling of sample plant material to chemical derivatization procedures such as silylation and methoxymation (necessary for gas chromatography only); choosing instrumentation settings and evaluating the resultant chromatogram in terms of both unlabeled and labeled peaks."

According to the news editors, the research concluded: "Furthermore, we describe how quantitative insights can be gained by estimating both benchmark and previously unknown fluxes from collected data sets."

For more information on this research see: Flux profiling of photosynthetic carbon metabolism in intact plants. Nature Protocols, 2014;9(8):1803-1824. Nature Protocols can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group -; Nature Protocols -

The news editors report that additional information may be obtained by contacting R. Heise, Max Planck Inst Mol Pflanzenphysiol, Potsdam, Germany. Additional authors for this research include S. Arrivault, M. Szecowka, T. Tohge, A. Nunes-Nesi, M. Stitt, Z. Nikoloski and A.R. Fernie (see also Life Science Research).

Keywords for this news article include: Europe, Potsdam, Germany, Life Science Research

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Source: Life Science Weekly

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