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Johns Hopkins University Reports Findings in Pharmaceutical and Biomedical Analysis [Dual quantification of dapivirine and maraviroc in...

September 3, 2014



Johns Hopkins University Reports Findings in Pharmaceutical and Biomedical Analysis [Dual quantification of dapivirine and maraviroc in cervicovaginal secretions from ophthalmic tear strips and polyester-based swabs via liquid ...]

By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Biotechnology are discussed in a new report. According to news reporting out of Baltimore, Maryland, by NewsRx editors, research stated, "Topical microbicidal agents are being actively pursued as a modality to prevent HIV viral transmission during sexual intercourse. Quantification of antiretroviral agents in specimen sources where antiviral activity is elicited is critical, and drug measurements in cervicovaginal fluid can provide key information on local drug concentrations."

Our news journalists obtained a quote from the research from Johns Hopkins University, "Two antiretroviral drugs, dapivirine and maraviroc, have gained interest as vaginal microbicidal agents, and rugged methods are required for their quantification in cervicovaginal secretions. Cervicovaginal fluid spiked with dapivirine and maraviroc were applied to ophthalmic tear strips or polyester-based swabs to mimic collection procedures used in clinical studies. Following sample extraction and the addition of isotopically labeled internal standards, samples were subjected to liquid chromatographic-tandem mass spectrometric (LC-MS/MS) analysis using a Waters BEH C8, 50 mm x 2.1 mm, 1.7 mu m particle size column, on an API 4000 mass analyzer operated in selective reaction monitoring mode. The method was validated according to FDA Bioanalytical Method Validation guidelines. Due to the disparate saturation capacity of the tested collection devices, the analytical measuring ranges for dapivirine and maravirocin cervicovaginal fluid on the ophthalmic tear strip were 0.05-25 ng/tear strip, and 0.025-25 ng/tear strip, respectively. As for the polyester-based swab, the analytical measuring ranges were 0.25-125 ng/swab for dapivirine and 0.125-125 ng/swab for maraviroc. Dilutional studies were performed for both analytes to extended ranges of 25,000 ng/tear strip and 11,250 ng/swab. Standard curves were generated via weighted (1/x(2)) linear or quadratic regression of calibrators. Precision, accuracy, stability and matrix effects studies were all performed and deemed acceptable according to the recommendations of the FDA Bioanalytical Method Validation guidelines. A rugged LC-MS/MS method for the dual quantification of dapivirine and maraviroc in cervicovaginal fluid using two unique collection devices has been developed and validated."

According to the news editors, the research concluded: "The described method meets the criteria to support large research trials."

For more information on this research see: Dual quantification of dapivirine and maraviroc in cervicovaginal secretions from ophthalmic tear strips and polyester-based swabs via liquid chromatographic-tandem mass spectrometric (LC-MS/MS) analysis. Journal of Pharmaceutical and Biomedical Analysis, 2014;98():407-416. Journal of Pharmaceutical and Biomedical Analysis can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Pharmaceutical and Biomedical Analysis - www.elsevier.com/wps/product/cws_home/525434)

Our news journalists report that additional information may be obtained by contacting T.L. Parsons, Johns Hopkins University, Dept. of Pathol, Baltimore, MD 21287, United States. Additional authors for this research include J.F. Emory, L.A. Seserko, W.S. Aung and M.A. Marzinke (see also Biotechnology).

Keywords for this news article include: Biotechnology, Maryland, Baltimore, United States, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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