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Findings on Biotechnology Reported by Investigators at Uppsala University (In situ generation of iminodiacetic acid groups on nanoporous alumina for...

September 2, 2014



Findings on Biotechnology Reported by Investigators at Uppsala University (In situ generation of iminodiacetic acid groups on nanoporous alumina for the reversible immobilization of enzymes and other biomolecules)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Technology. According to news originating from Uppsala, Sweden, by NewsRx correspondents, research stated, "Nanoporous alumina membranes were silanized with aminopropylsilane and iminodiacetic acid (IDA) groups were generated in situ by reaction with iodoacetate. The membranes were mounted in standard filter holders, connected to a HPLC system and saturated with selected metal ions."

Our news journalists obtained a quote from the research from Uppsala University, "Cu(II) allowed the capture of chicken muscle lactate dehydrogenase with such stability, repeatability and reproducibility that Michaelis-Menten kinetics could be studied. The IDA surface was stable for months and could be depleted and regenerated with metal ions multiple times without appreciable loss of capacity."

According to the news editors, the research concluded: "The binding of lactate dehydrogenase influenced the backpressure to the extent that could be expected for a monolayer according to Poiseuilles law."

For more information on this research see: In situ generation of iminodiacetic acid groups on nanoporous alumina for the reversible immobilization of enzymes and other biomolecules. Biotechnology Letters, 2014;36(9):1819-1825. Biotechnology Letters can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Biotechnology Letters - www.springerlink.com/content/0141-5492/)

The news correspondents report that additional information may be obtained from L. Berglin, Uppsala University, Dept. of Chem BMC, Uppsala, Sweden. Additional authors for this research include M. Kjellander and G. Johansson (see also Technology).

Keywords for this news article include: Sweden, Europe, Uppsala, Technology, Dehydrogenase, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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