Findings from University of Cairo Update Understanding of Acinetobacter baumannii (A sensitive colorimetric assay for identification of Acinetobacter baumannii using unmodified gold nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteobacteria. According to news originating from Cairo, Egypt, by NewsRx correspondents, research stated, "Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals."
Our news journalists obtained a quote from the research from the University of Cairo, "Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction using boiling method and PCR amplification of the 23S-16S rRNA intergenic spacer sequences (ITS). AuNPs were synthesized using citrate reduction method. Detection and optimization of Ac. baumannii amplicons using unmodified spherical AuNPs were performed using species-specific DNA oligonucleotide. The nano-gold assay was able to colorimetrically detect and distinguish Ac. baumannii from other Gram-negative bacteria. The turnaround time of the assay is about 2 h including sample treatment and amplification. The assay detection limit is 0.8125 ng of DNA. The developed colorimetric assay is sensitive, fast and reliable and can be used for identification of Ac. baumannii. Significance and Impact of the Study: There is a need to develop robust, rapid, and specific methods for detection of Ac. baumannii isolated from clinical specimens."
According to the news editors, the research concluded: "The developed nanogold assay prototype allows sensitive, specific and rapid detection of amplified DNA of A. baumannii and represents a reliable diagnostic tool to aid routine laboratory identification of this pathogen."
For more information on this research see: A sensitive colorimetric assay for identification of Acinetobacter baumannii using unmodified gold nanoparticles. Journal of Applied Microbiology, 2014;117(2):465-471. Journal of Applied Microbiology can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Applied Microbiology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2672)
The news correspondents report that additional information may be obtained from M.A.F. Khalil, Cairo University, Dept. of Microbiol & Immunol, Fac Pharm, Cairo, Egypt. Additional authors for this research include H.M.E. Azzazy, A.S. Attia and A.G.M. Hashem (see also Proteobacteria).
Keywords for this news article include: Cairo, Egypt, Africa, Nanoparticle, Moraxellaceae, Nanotechnology, Gammaproteobacteria, Emerging Technologies, Gram-Negative Bacteria, Acinetobacter baumannii, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci
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