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Data on Estradiol Congeners Reported by Researchers at University of British Columbia (Localization of Cytochrome P450 and Related Enzymes in Adult...

September 3, 2014



Data on Estradiol Congeners Reported by Researchers at University of British Columbia (Localization of Cytochrome P450 and Related Enzymes in Adult Rat Testis and Downregulation by Estradiol and Bisphenol A)

By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Hormones are discussed in a new report. According to news reporting from Vancouver, Canada, by NewsRx journalists, research stated, "There is a growing body of evidence that exposure to endocrine disrupting chemicals and to estrogenic compounds in particular can affect the testis and male fertility. In the present study, the constitutive expression of steroidogenic and non-steroidogenic cytochrome P450 (CYP) and related enzymes in adult rat testis, and their regulation by estradiol and bisphenol A, were investigated."

The news correspondents obtained a quote from the research from the University of British Columbia, "CYP1B1, CYP2A1, NADPH-cytochrome P450 oxidoreductase (POR) and microsomal epoxide hydrolase (mEH) proteins, together with CYP17A1 and 3 beta-hydroxysteroid dehydrogenase (HSD3B), were detected by immunoblot analysis in testicular microsomes prepared from untreated adult Sprague Dawley rats. In contrast, CYP1A, CYP2B, CYP2E, CYP2D, CYP2C, CYP3A, and CYP4A enzymes were not detected. Immunofluorescence staining of cryosections of perfusion-fixed testes showed that CYP1B1, CYP2A1, CYP17A1, and HSD3B were expressed exclusively or mainly in interstitial cells, whereas mEH and POR protein staining was detected both in interstitial cells and in seminiferous tubules. Testicular CYP1B1 and CYP2A1 protein levels were decreased following treatment of adult rats with estradiol benzoate at 0.004, 0.04, 0.4, or 4 mu mol/kg/day or bisphenol A at 400 or 800 mu mol/kg/day, for 14 days, whereas expression of HSD3B was unaffected."

According to the news reporters, the research concluded: "Testicular CYP17A1, POR, and mEH protein expression was also downregulated at the three highest dosages of estradiol benzoate and at both dosages of bisphenol A. The present study is the first to establish the cellular localization of CYP1B1, mEH, and POR in rat testis and to demonstrate the suppressive effect of bisphenol A on testicular CYP1B1, CYP2A1, mEH, and POR protein levels."

For more information on this research see: Localization of Cytochrome P450 and Related Enzymes in Adult Rat Testis and Downregulation by Estradiol and Bisphenol A. Toxicological Sciences, 2014;140(1):26-39. Toxicological Sciences can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Toxicological Sciences - toxsci.oxfordjournals.org)

Our news journalists report that additional information may be obtained by contacting R.R. Gilibili, University of British Columbia, Fac Med, Dept. of Cellular & Physiol Sci, Vancouver, BC V6T 1Z3, Canada. Additional authors for this research include A.W. Vogl, T.K.H. Chang and S.M. Bandiera (see also Hormones).

Keywords for this news article include: Canada, Vancouver, Cytochromes, Hemeproteins, British Columbia, Gonadal Hormones, Estradiol Congeners, Enzymes and Coenzymes, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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