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Recent Findings from University of Pittsburgh School of Medicine Provide New Insights into Science and Technology (Transmission electron microscopy...

August 29, 2014



Recent Findings from University of Pittsburgh School of Medicine Provide New Insights into Science and Technology (Transmission electron microscopy as a tool for nanocrystal characterization pre- and post-injector)

By a News Reporter-Staff News Editor at Science Letter -- Current study results on Science have been published. According to news reporting from Pittsburgh, Pennsylvania, by NewsRx journalists, research stated, "Recent advancements at the Linac Coherent Light Source X-ray free-electron laser (XFEL) enabling successful serial femtosecond diffraction experiments using nanometre-sized crystals (NCs) have opened up the possibility of X-ray structure determination of proteins that produce only submicrometre crystals such as many membrane proteins. Careful crystal pre-characterization including compatibility testing of the sample delivery method is essential to ensure efficient use of the limited beamtime available at XFEL sources."

The news correspondents obtained a quote from the research from the University of Pittsburgh School of Medicine, "This work demonstrates the utility of transmission electron microscopy for detecting and evaluating NCs within the carrier solutions of liquid injectors. The diffraction quality of these crystals may be assessed by examining the crystal lattice and by calculating the fast Fourier transform of the image. Injector reservoir solutions, as well as solutions collected post-injection, were evaluated for three types of protein NCs (i) the membrane protein PTHR1, (ii) the multi-protein complex Pol II-GFP and (iii) the soluble protein lysozyme."

According to the news reporters, the research concluded: "Our results indicate that the concentration and diffraction quality of NCs, particularly those with high solvent content and sensitivity to mechanical manipulation may be affected by the delivery process."

For more information on this research see: Transmission electron microscopy as a tool for nanocrystal characterization pre- and post-injector. Philosophical Transactions, 2014;369(1647):20130322 (see also Science).

Our news journalists report that additional information may be obtained by contacting H.P. Stevenson, Dept. of Structural Biology, University of Pittsburgh School of Medicine, 1040 Biomedical Science Tower 3, Pittsburgh, PA 15260, United States. Additional authors for this research include D.P. DePonte, A.M. Makhov, J.F. Conway, O.B. Zeldin, S. Boutet, G. Calero and A.E Cohen.

Keywords for this news article include: Science, Pittsburgh, Pennsylvania, United States, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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