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Study Results from Third Military Medical University Update Understanding of Spermatozoa (The protective effect of autophagy on mouse spermatocyte...

August 26, 2014



Study Results from Third Military Medical University Update Understanding of Spermatozoa (The protective effect of autophagy on mouse spermatocyte derived cells exposure to 1800 MHz radiofrequency electromagnetic radiation)

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Spermatozoa are discussed in a new report. According to news reporting originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "The increasing exposure to radiofrequency (RF) radiation emitted from mobile phone use has raised public concern regarding the biological effects of RF exposure on the male reproductive system. Autophagy contributes to maintaining intracellular homeostasis under environmental stress."

Our news editors obtained a quote from the research from Third Military Medical University, "To clarify whether RF exposure could induce autophagy in the spermatocyte, mouse spermatocyte-derived cells (GC-2) were exposed to 1800 MHz Global System for Mobile Communication (GSM) signals in GSM-Talk mode at specific absorption rate (SAR) values of 1 w/kg, 2 w/kg or 4 w/kg for 24 h, respectively. The results indicated that the expression of LC3-II increased in a dose- and time-dependent manner with RF exposure, and showed a significant change at the SAR value of 4 w/kg. The autophagosome formation and the occurrence of autophagy were further confirmed by GFP-LC3 transient transfection assay and transmission electron microscopy (TEM) analysis. Furthermore, the conversion of LC3-I to LC3-II was enhanced by co-treatment with Chloroquine (CQ), indicating autophagic flux could be enhanced by RF exposure. Intracellular ROS levels significantly increased in a dose- and time-dependent manner after cells were exposed to RF. Pretreatment with anti-oxidative NAC obviously decreased the conversion of LC3-I to LC3-II and attenuated the degradation of p62 induced by RF exposure. Meanwhile, phosphorylated extracellular-signal-regulated kinase (ERK) significantly increased after RF exposure at the SAR value of 2 w/kg and 4 w/kg. Moreover, we observed that RF exposure did not increase the percentage of apoptotic cells, but inhibition of autophagy could increase the percentage of apoptotic cells. These findings suggested that autophagy flux could be enhanced by 1800 MHz GSM exposure (4 w/kg), which is mediated by ROS generation."

According to the news editors, the research concluded: "Autophagy may play an important role in preventing cells from apoptotic cell death under RF exposure stress."

For more information on this research see: The protective effect of autophagy on mouse spermatocyte derived cells exposure to 1800 MHz radiofrequency electromagnetic radiation. Toxicology Letters, 2014;228(3):216-224. Toxicology Letters can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier - www.elsevier.com; Toxicology Letters - www.elsevier.com/wps/product/cws_home/505519)

The news editors report that additional information may be obtained by contacting K.J. Liu, Third Military Medical University, Inst Toxicol, Coll Prevent Med, Chongqing 400038, People's Republic of China. Additional authors for this research include G.W. Zhang, Z. Wang, Y. Liu, J.Y. Dong, X.M. Dong, J.Y. Liu, J. Cao, L. Ao and S.X. Zhang (see also Spermatozoa).

Keywords for this news article include: Asia, Chongqing, Spermatozoa, Spermatocytes, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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