Studies from Third Military Medical University Describe New Findings in Xenografts (Thiol-PEG-carboxyl-stabilized Fe?O ?/Au nanoparticles targeted to CD105: synthesis, characterization and application in MR imaging of tumor angiogenesis)
By a News Reporter-Staff News Editor at Women's Health Weekly -- Current study results on Biotechnology have been published. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "To detect tumor angiogenesis in tumor-bearing mice using thiol-PEG-carboxyl-stabilized Fe2O3/Au nanoparticles targeted to CD105 on magnetic resonance imaging (MRI). Fe2O3/Au nanoparticles (hybrids) were prepared by reducing Au(3+) on the surface of Fe2O3 nanoparticles."
Our news journalists obtained a quote from the research from Third Military Medical University, "Hybrids were stabilized with thiol-PEG-carboxyl via the Au-S covalent bond, and further conjugated with anti-CD105 antibodies through amide linkages. Characteristics of the hybrid-PEG-CD105 nanoparticles were evaluated. Using these nanoparticles, the labeling specificity of human umbilical vein endothelial cells (HUVECs) was evaluated in vitro. MRI T2-weighted images were obtained at different time points after intravenous administration of the hybrid-PEG-CD105 nanoparticles in the tumor-bearing mice. After MR imaging, the breast cancer xenografts were immediately resected for immunohistochemistry staining and Prussian blue staining to measure the tumor microvessel density (MVD) and evaluate the labeling of blood microvessels by the hybrid-PEG-CD105 nanoparticles in vivo. The mean diameter of the hybrid-PEG-CD105 nanoparticles was 56.6 ± 8.0 nm, as measured by transmission electron microscopy (TEM). Immune activity of the hybrid-PEG-CD105 nanoparticles was 53% of that of the anti-CD105 antibody, as detected by enzyme-linked immunosorbent assay (ELISA). The specific binding of HUVECs with the hybrid-PEG-CD105 nanoparticles was proved by immunostaining and Prussian blue staining in vitro. For breast cancer xenografts, the combination of the hybrid-PEG-CD105 nanoparticles with blood microvessels was detectable by MRI after 60 min administration of the contrast agent. The T2* relative signal intensity (SIR) was positively correlated with the tumor MVD (R(2)=0.8972). Anti-CD105 antibody-coupled, thiol-PEG-carboxyl-stabilized core-shell Fe2O3/Au nanoparticles can efficiently target CD105 expressed by HUVECs."
According to the news editors, the research concluded: "Furthermore, the hybrid-PEG-CD105 nanoparticles can be used to detect tumor angiogenesis in vivo."
For more information on this research see: Thiol-PEG-carboxyl-stabilized Fe?O ?/Au nanoparticles targeted to CD105: synthesis, characterization and application in MR imaging of tumor angiogenesis. European Journal of Radiology, 2014;83(7):1190-8. (Elsevier - www.elsevier.com; European Journal of Radiology - www.elsevier.com/wps/product/cws_home/506039)
Our news journalists report that additional information may be obtained by contacting S. Zhang, Dept. of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, People's Republic of China. Additional authors for this research include M. Gong, D. Zhang, H. Yang, F. Gao and L. Zou (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Chongqing, Chemicals, Chemistry, Xenograft, Angiogenesis, Nanoparticle, Prussian Blue, Nanotechnology, Xenotransplantion, Emerging Technologies, People's Republic of China.
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