Studies from Catalan Institution for Research and Advanced Studies (ICREA) Further Understanding of Peptide Hormones (Supramolecular Amperometric Immunosensor for Detection of Human Chorionic Gonadotropin)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Peptide Proteins. According to news reporting originating in Barcelona, Spain, by NewsRx journalists, research stated, "Human chorionic gonadotropin (hCG) is a hormone produced in high concentrations through the placental trophoblasts and is used for the detection of pregnancy and certain diseases. Here we explored a supramolecular strategy for the potentially substrateless amperometric detection of hCG."
The news reporters obtained a quote from the research from Catalan Institution for Research and Advanced Studies (ICREA), "A carboxymethylcellulose (CMC) carrier was synthesised and trifunctionalised with anti-beta hCG antibody, horse radish peroxidase (HRP) and ferrocene (Fc) moieties. The ferrocene was used to house the functionalised CMC within the cavities of electrode surface immobilised cyclodextrin, via host-guest interactions. hCG was detected via a sandwich format, forming an immunocomplex between the surface immobilised antibody and a glucose oxidase/lactate oxidase labelled secondary antibody. Following formation of the immunocomplex, lactate/glucose, which would typically be present in serum/urine samples, was added and the hydrogen peroxide formed detected at the electrode surface via the HRP-Fc enzyme-mediator couple."
According to the news reporters, the research concluded: "The work reported demonstrates a potential supramolecular platform for the detection of targets in blood/urine samples using endogenous substrates."
For more information on this research see: Supramolecular Amperometric Immunosensor for Detection of Human Chorionic Gonadotropin. Electroanalysis, 2014;26(7):1481-1487. Electroanalysis can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Electroanalysis - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4109)
Our news correspondents report that additional information may be obtained by contacting M. Ortiz, ICREA, Barcelona 08010, Spain. Additional authors for this research include L. Wilson, M.L. Botero, P. Baker, E. Iwuoha, A. Fragoso and C.K. O'Sullivan (see also Peptide Proteins).
Keywords for this news article include: Antibodies, Spain, Europe, Oxidase, Barcelona, Immunology, Blood Proteins, Nanotechnology, Supramolecular, Immunoglobulins, Peptide Hormones, Peptide Proteins, Placental Hormones, Pregnancy Proteins, Emerging Technologies, Enzymes and Coenzymes, Chorionic Gonadotropin
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