Researchers from University of Torino Report Recent Findings in Myeloid Cells (Imogolite: An Aluminosilicate Nanotube Endowed with Low Cytotoxicity and Genotoxicity)
By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on Myeloid Cells is now available. According to news reporting originating from Turin, Italy, by NewsRx correspondents, research stated, "High-aspect-ratio nanomaterials (HARN) (typically; single-walled carbon nanotubes (SWCNT) or multiwalled carbon nanotubes (MWCNT)) impair airway barrier function and are toxic to macrophages. Here, we assess the biological effects of nanotubes of irnogolite (INT), a hydrated aluminosilicate [(OH)(3)Al2O3SiOH] occurring as single-walled NT, on murine macrophages and human airway epithelial cells."
Our news editors obtained a quote from the research from the University of Torino, "Cell viability was assessed with resazurin. RT-PCR was used to study the expression of Nos2 and Arg1, markers of classical or alternative macrophage activation, respectively, and nitrite concentration in the medium was determined to assess NO production. Epithelial barrier integrity was evaluated from the trans-epithelial electrical resistance (TEER). Potential genotoxicity of INT was assessed with comet and cytokinesis-block micronucleus cytome assays. Compared to MWCNT and SWCNT, INT caused much smaller effects on RAW264.7 and MH-S macrophage viability. The incubation of macrophages with INT at doses as high as 120 mu g/cm(2) for 72 h did not alter either Nos2 or ArgI expression nor did it increase NO production, whereas IL6 was induced in RAW264.7 cells but not in MH-S cells. INT did not show any genotoxic effect in RAW264.7 and A549 cells except for a decrease in DNA integrity observed in epithelial A549 cells after treatment with the highest dose (80 mu g/cm(2))."
According to the news editors, the research concluded: "No significant change in permeability was recorded in Calu-3 epithelial cell monolayers exposed to INT, whereas comparable doses of both SWCNT and MWCNT lowered TEER Thus, in spite of their fibrous nature, INT appear not to be markedly toxic for in vitro models of lung-blood barrier cells."
For more information on this research see: Imogolite: An Aluminosilicate Nanotube Endowed with Low Cytotoxicity and Genotoxicity. Chemical Research in Toxicology, 2014;27(7):1142-1154. Chemical Research in Toxicology can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Chemical Research in Toxicology - www.pubs.acs.org/journal/crtoec)
The news editors report that additional information may be obtained by contacting B.M. Rotoli, Univ Torino, G Scansetti Interdept Center Studies Asbesto & Other, I-10125 Turin, Italy. Additional authors for this research include P. Guidi, B. Bonelli, M. Bernardeschi, M.G. Bianchi, S. Esposito, G. Frenzilli, P. Lucchesi, M. Nigro, V. Scarcelli, M. Tomatis, P.P. Zanello, B. Fubini, O. Bussolati and E. Bergamaschi (see also Myeloid Cells).
Keywords for this news article include: Turin, Italy, Europe, Immunology, Macrophages, Myeloid Cells, Connective Tissue Cells, Mononuclear Phagocyte System
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