Researchers from Ben-Gurion University of the Negev Describe Findings in Proteomics (Amplification of Single Molecule Trans location Signal Using beta-Strand Peptide Functionalized Nanopores)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Proteomics is now available. According to news originating from Beer Sheva, Israel, by NewsRx correspondents, research stated, "Changes in ionic current flowing through nanopores due to binding or translocation of single biopolymer molecules enable their detection and characterization. It is, however, much more challenging to detect small molecules due to their rapid and small signal signature."
Our news journalists obtained a quote from the research from the Ben-Gurion University of the Negev, "Here we demonstrate the use of de novo designed peptides for functionalization of nanopores that enable the detection of a small analytes at the single molecule level. The detection relies on cooperative peptide conformational change that is induced by the binding of the small molecule to a receptor domain on the peptide. This change results in alteration of the nanopore effective diameter and hence induces current perturbation signal. On the basis of this approach, we demonstrate here the detection of diethyl 4-nitrophenyl phosphate (paraoxon), a poisonous organophosphate molecule. Paraoxon binding is induced by the incorporation of the catalytic triad of acetylcholine esterase in the hydrophilic domain of a short amphiphilic peptide and promotes beta-sheet assembly of the peptide both in solution and for peptide molecules immobilized on solid surfaces. Nanopores coated with this peptide allowed the detection of paraoxon at the single molecule level revealing two binding arrangements. This unique approach, hence, provides the ability to study interactions of small molecules with the corresponding engineered receptors at the single molecule level."
According to the news editors, the research concluded: "Furthermore, the suggested versatile platform may be used for the development of highly sensitive small analytes sensors."
For more information on this research see: Amplification of Single Molecule Trans location Signal Using beta-Strand Peptide Functionalized Nanopores. ACS Nano, 2014;8(7):6822-6832. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
The news correspondents report that additional information may be obtained from Y. Liebes-Peer, Ben Gurion University of the Negev, Ilze Katz Inst Nanoscale Technol, IL-84105 Beer Sheva, Israel. Additional authors for this research include H. Rapaport and N. Ashkenasy (see also Proteomics).
Keywords for this news article include: Asia, Israel, Peptides, Proteins, Beer Sheva, Proteomics
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