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Investigators at National Taiwan University Report New Data on Aromatic Polycyclic Hydrocarbons (NMR- and MS-based metabolomics: various organ...

August 26, 2014



Investigators at National Taiwan University Report New Data on Aromatic Polycyclic Hydrocarbons (NMR- and MS-based metabolomics: various organ responses following naphthalene intervention)

By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Aromatic Polycyclic Hydrocarbons are presented in a new report. According to news originating from Taipei, Taiwan, by NewsRx correspondents, research stated, "Naphthalene, a polycyclic aromatic hydrocarbon, is a ubiquitous environmental pollutant capable of causing illness. In this study, we deconvoluted the metabolites related to naphthalene intervention in various organs by using nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)."

Our news journalists obtained a quote from the research from National Taiwan University, "Male ICR mice were intraperitoneally dosed with olive oil (vehicle), and a low dose and a high dose (100 and 200 mg kg(-1) body wt, respectively) of naphthalene. After 48 h, the lungs, liver, and kidneys were collected for analysing the metabolic responses. The metabolites were extracted and non-targeted profiled using NMR. Low NMR resolution limited the identification of the hydrophobic metabolites. Therefore, LC-MS/MS-based focus lipidomics was applied to profile phosphorylcholine-containing lipids and sphingolipids. Chemometric analysis revealed that succinate and lactate were significantly increased in the lungs, suggesting that energy metabolisms and antioxidation were increased following naphthalene treatment. In the liver, anti-oxidative stress-related metabolites increased, enabling the oxidative stress during naphthalene biotransformation and detoxification to be overcome. The elevation of glutathione protected kidneys from reactive-naphthalene-metabolite-induced injury. Significant alteration of hydrophobic metabolites (membrane constituents) revealed lung and liver were the target organs of naphthalene treatment. MS data demonstrated that phosphatidylcholine (PC) and ceramide species were significantly altered in the lungs and liver, whereas only PC was observed in the kidneys. Elevated numbers of unsaturated bonds and fatty acyl chains in both ceramides and PCs were determined to reduce cellular membrane rigidity and facilitating the trafficking of recovery elements into the cell for rejuvenation."

According to the news editors, the research concluded: "To conclude, the complementary results of NMR-and MS-based metabolomics enabled the characterization of naphthalene-induced changes in various organs."

For more information on this research see: NMR- and MS-based metabolomics: various organ responses following naphthalene intervention. Molecular Biosystems, 2014;10(7):1918-31. (Royal Society of Chemistry - www.rsc.org/; Molecular Biosystems - pubs.rsc.org/en/journals/journalissues/mb)

The news correspondents report that additional information may be obtained from Y.S. Ling, Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No1, Sec 1, Jen-ai Rd, Taipei 100, Taiwan. Additional authors for this research include H.J. Liang, M.H. Chung, M.H. Lin and C.Y Lin (see also Aromatic Polycyclic Hydrocarbons).

Keywords for this news article include: Asia, Taipei, Taiwan, Naphthalenes, Organic Chemicals, Aromatic Polycyclic Hydrocarbons.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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