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Findings from Vanderbilt University Reveals New Findings on Immunology (Validation of a multiplex electrochemiluminescent immunoassay platform in...

August 29, 2014



Findings from Vanderbilt University Reveals New Findings on Immunology (Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Fresh data on Immunology are presented in a new report. According to news originating from Nashville, Tennessee, by NewsRx correspondents, research stated, "Despite the widespread use of multiplex immunoassays, there are very few scientific reports that test the accuracy and reliability of a platform prior to publication of experimental data. Our laboratory has previously demonstrated the need for new assay platform validation prior to use of biologic samples from large studies in order to optimize sample handling and assay performance."

Our news journalists obtained a quote from the research from Vanderbilt University, "In this study, our goal was to test the accuracy and reproducibility of an electrochemiluminescent multiplex immunoassay platform (Mesa Scale Discovery, MSD ) and compare this platform to validated, singleplex immunoassays (R&D Systems ) using actual study subject (human plasma and mouse bronchoalveolar lavage fluid (BAL) and plasma) samples. We found that the MSD platform performed well on intra- and inter-assay comparisons, spike and recovery and cross-platform comparisons. The mean intra-assay CV% and range for MSD were 3.49 (0.0-10.4) for IL-6 and 2.04 (0.1-7.9) for IL-8. The correlation between values for identical samples measured on both MSD and R&D was R = 0.97 for both analytes. The mouse MSD assay had a broader range of CV% with means ranging from 9.5 to 28.5 depending on the analyte. The range of mean CV% was similar for single plex ELISAs at 43-23.7 depending on the analyte. Regardless of species or sample type, CV% was more variable at lower protein concentrations. In conclusion, we validated a multiplex electrochemiluminescent assay system and found that it has superior test characteristics in human plasma compared to mouse BALF and plasma."

According to the news editors, the research concluded: "Both human and MSD assays compared favorably to well-validated singleplex ELISAs. ."

For more information on this research see: Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples. Journal of Immunological Methods, 2014;408():13-23. Journal of Immunological Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Immunological Methods - www.elsevier.com/wps/product/cws_home/506022)

The news correspondents report that additional information may be obtained from J.A. Bastarache, Vanderbilt University, Sch Med, Dept. of Biostat, Nashville, TN 37232, United States. Additional authors for this research include T. Koyama, N.E. Wickersham and L.B. Ware (see also Immunology).

Keywords for this news article include: Nashville, Tennessee, Immunology, United States, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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