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Findings from H.B. Costa and Co-Authors Provide New Insights into Agribusiness (A new procedure based on column chromatography to purify bromelain by...

August 28, 2014



Findings from H.B. Costa and Co-Authors Provide New Insights into Agribusiness (A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies)

By a News Reporter-Staff News Editor at Agriculture Week -- Investigators discuss new findings in Agribusiness. According to news reporting originating in Vitoria, Brazil, by VerticalNews journalists, research stated, "Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others."

The news reporters obtained a quote from the research, "The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed."

According to the news reporters, the research concluded: "Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues."

For more information on this research see: A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. Industrial Crops and Products, 2014;59():163-168. Industrial Crops and Products can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Industrial Crops and Products - www.elsevier.com/wps/product/cws_home/522825)

Our news correspondents report that additional information may be obtained by contacting H.B. Costa, Inst Capixaba Pesquisa Assistencia Tecn & Extensa, BR-29052010 Vitoria, ES, Brazil. Additional authors for this research include P.M.B. Fernandes, W. Romao and J.A. Ventura.

Keywords for this news article include: Brazil, Vitoria, Agribusiness, Agricultural, South America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Agriculture Week


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