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Data on Bioscience and Bioengineering Detailed by Researchers at Keio University [Time-resolved metabolomics of a novel trebouxiophycean alga using...

August 26, 2014

Data on Bioscience and Bioengineering Detailed by Researchers at Keio University [Time-resolved metabolomics of a novel trebouxiophycean alga using (13)CO2 feeding]

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Life Science Research is the subject of a report. According to news originating from Tsuruoka, Japan, by NewsRx correspondents, research stated, "Oil-rich algae are potentially promising as next-generation biofuel feedstock. However, the productivity of oil needs to be improved for industrial use."

Our news journalists obtained a quote from the research from Keio University, "The biosynthesis of oil and its control mechanism have not been characterized in any algae, and understanding the metabolic network is vital to achieve the precise engineering of algae metabolic pathways. 'Pseudochoricystis ellipsoidea' MBIC 11204, a novel microalgal strain, accumulates a large amount of lipids in nitrogen-deficient conditions. In this study, 'P. ellipsoidea' was grown in flat flasks with continuous illumination and aeration with 1% CO2 at 25C. During the exponential growth phase, CO2 was switched to (13)C-labeled CO2 and samples were collected for time-course experiments. Seventy-eight pairs of unlabeled and uniformly (13)C-labeled metabolites were quantified using a capillary electrophoresis-and liquid chromatography-mass spectrometry for ionic primary metabolites and lipids, respectively. The (13)C-exchange indices of the metabolites were calculated from a concentration of unlabeled and uniformly-labeled metabolites. A hierarchical clustering analysis of the dynamics of the indices revealed 4 characteristic clusters, two of which represented rapidly-labeled metabolites, mainly composed of primary metabolites, while the two other clusters represented slowly-labeled metabolites, mainly composed of lipids. Moreover, the labeling order of these clusters was mainly matched to the metabolic process of Chlamydomonas reinhardtii, a model organism of green algae. In TCA cycle, anomalistically different of the labeling order was found."

According to the news editors, the research concluded: "To the author's knowledge, this study for the first time in literature, characterize the features of global metabolism in 'P."

For more information on this research see: Time-resolved metabolomics of a novel trebouxiophycean alga using (13)CO2 feeding. Journal of Bioscience and Bioengineering, 2013;116(3):408-15. (Elsevier -; Journal of Bioscience and Bioengineering -

The news correspondents report that additional information may be obtained from T. Ito, Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata 997-0052, Japan. Additional authors for this research include M. Sugimoto, Y. Toya, Y. Ano, N. Kurano, T. Soga and M. Tomita (see also Life Science Research).

Keywords for this news article include: Asia, Japan, Tsuruoka, Life Science Research.

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Source: Life Science Weekly

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