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Study Findings on Nanoparticles Are Outlined in Reports from University of Munster (Affinity Protein-Based FRET Tools for Cellular Tracking of...

August 22, 2014



Study Findings on Nanoparticles Are Outlined in Reports from University of Munster (Affinity Protein-Based FRET Tools for Cellular Tracking of Chitosan Nanoparticles and Determination of the Polymer Degree of Acetylation)

By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Nanoparticles. According to news originating from Munster, Germany, by NewsRx correspondents, research stated, "Chitosan (CS) is a family of linear polysaccharides with diverse applications in medicine, agriculture, and industry. Its bioactive properties are determined by parameters such as the degree of acetylation (DA), but current techniques to measure the DA are laborious and require large amounts of substrate and sophisticated equipment."

Our news journalists obtained a quote from the research from the University of Munster, "It is also challenging to monitor the fate of chitosan-based nanopartides (CS-NPs) in vitro because current tools cannot measure their enzymatic or chemical degradation. We have developed a method based on the Forster resonance energy transfer (FRET) that occurs between two independent fluorescent proteins fused to a CS-binding domain, who interact with CS polymers or CS-NPs. We used this approach to calibrate a simple and rapid analytical method that can determine the DA of CS substrates. We showed unequivocally that FRET occurs on the surface of CS-NPs and that the FRET signal is quenched by enzymatic degradation of the CS substrate."

According to the news editors, the research concluded: "Finally, we provide in vitro proof-of-concept that these approaches can be used to label CS-NPs and colocalize them following their interactions with mammalian cells."

For more information on this research see: Affinity Protein-Based FRET Tools for Cellular Tracking of Chitosan Nanoparticles and Determination of the Polymer Degree of Acetylation. Biomacromolecules, 2014;15(7):2532-2539. Biomacromolecules can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biomacromolecules - www.pubs.acs.org/journal/bomaf6)

The news correspondents report that additional information may be obtained from J.P. Fuenzalida, University of Munster, IBBP, D-48149 Munster, Germany. Additional authors for this research include T. Weikert, S. Hoffmann, C. Vila-Sanjurjo, B.M. Moerschbacher, F.M. Goycoolea and S. Kolkenbrock (see also Nanoparticles).

Keywords for this news article include: Europe, Munster, Germany, Nanotechnology, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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