Investigators from Hunan University Release New Data on Molecular Engineering (Molecular Engineering of a TBET-Based Two-Photon Fluorescent Probe for Ratiometric Imaging of Living Cells and Tissues)
By a News Reporter-Staff News Editor at Journal of Engineering -- Investigators discuss new findings in Molecular Engineering. According to news originating from Changsha, People's Republic of China, by VerticalNews correspondents, research stated, "In contrast to one-photon microscopy, two-photon probe-based fluorescent imaging can provide improved three-dimensional spatial localization and increased imaging depth. Consequently, it has become one of the most attractive techniques for studying biological events in living cells and tissues."
Our news journalists obtained a quote from the research from Hunan University, "However, the quantitation of these probes is primarily based on single-emission intensity change, which tends to be affected by a variety of environmental factors. Ratiometric probes, on the other hand, can eliminate these interferences by the built-in correction of the dual emission bands, resulting in a more favorable system for imaging living cells and tissues. Herein, for the first time, we adopted a through-bond energy transfer (TBET) strategy to design and synthesize a small molecular ratiometric two-photon fluorescent probe for imaging living cells and tissues in real time. Specifically, a two-photon fluorophore (D-pi-A-structured naphthalene derivative) and a rhodamine B fluorophore are directly connected by electronically conjugated bond to form a TBET probe, or Np-Rh, which shows a target-modulated ratiometric two-photon fluorescence response with highly efficient energy transfer (93.7%) and two well-resolved emission peaks separated by 100 nm."
According to the news editors, the research concluded: "This novel probe was then applied for two-photon imaging of living cells and tissues and showed high ratiometric imaging resolution and deep-tissue imaging depth of 180 mu m, thus demonstrating its practical application in biological systems."
For more information on this research see: Molecular Engineering of a TBET-Based Two-Photon Fluorescent Probe for Ratiometric Imaging of Living Cells and Tissues. Journal of the American Chemical Society, 2014;136(28):9838-9841. Journal of the American Chemical Society can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
The news correspondents report that additional information may be obtained from L.Y. Zhou, Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Collaborat Innovat Center Mol Engn & Theranost, Coll BiolColl Chem & Chem EngnMol Sci & Biomed, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include X.B. Zhang, Q.Q. Wang, Y.F. Lv, G.J. Mao, A.L. Luo, Y.X. Wu, Y. Wu, J. Zhang and W.H. Tan.
Keywords for this news article include: Asia, Changsha, Nanotechnology, Emerging Technologies, Molecular Engineering, People's Republic of China
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