Studies from University of Tokyo Have Provided New Data on Bioscience and Bioengineering [Image-based evaluations of distribution and cytotoxicity of Irinotecan (CPT-11) in a multi-compartment micro-cell coculture device]
By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Bioengineering. According to news originating from Tokyo, Japan, by NewsRx correspondents, research stated, "We recently developed a polydimethylsiloxane (PDMS)-based three-compartment microfluidic cocultivation device enabling real-time interactions of different cell populations as an advanced physiologically-relevant cell-based assay. This device had valves and small magnetic stirrer-based internal pumps for easy and flexible perfusion operations."
Our news journalists obtained a quote from the research from the University of Tokyo, "In this study, we applied this device for the evaluation of Irinotecan (CPT-11) toxicity to the lung, because it is detoxified by the liver and accumulated in the fat in humans. We successfully cultured representative three different tissue model cells in each compartment under the individual culture conditions and also in entire perfusion. Growth inhibition of rat lung epithelial cell line L-2, was measured when administered with 50 mu M CPT-11 under various cocultivation conditions with respect to the presences and absence of primary rat hepatocytes (liver tissue model) and adipocyte-like cells (fat tissue model) induced from a mouse fibroblast cell line, 313-Ll. Although CPT-11 showed moderate toxicity to the pure culture of L-2 cells in the device after 72 h of perfusion culture, this was lowered mainly in the presence of the liver tissue. Inhibition of the L-2 cell growth agreed with the area under curve (AUC) values obtained from fluorescent image-based analyses in each compartment."
According to the news editors, the research concluded: "These results demonstrate that developed simple and flexible microfluidic cocultivation device, with appropriate image-based analyses, can be used in evaluating toxicokinetic behaviors of drug candidates in systemic levels."
For more information on this research see: Image-based evaluations of distribution and cytotoxicity of Irinotecan (CPT-11) in a multi-compartment micro-cell coculture device. Journal of Bioscience and Bioengineering, 2014;117(6):756-762. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news correspondents report that additional information may be obtained from H. Nakayama, University of Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan. Additional authors for this research include H. Kimura, T. Fujii and Y. Sakai (see also Bioengineering).
Keywords for this news article include: Asia, Tokyo, Japan, Bioengineering
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