Studies from Purdue University Yield New Information about Polyphosphates (Metabolic engineering of monoterpene biosynthesis in tomato fruits via introduction of the non-canonical substrate neryl diphosphate)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Polyphosphates is now available. According to news reporting from West Lafayette, Indiana, by NewsRx journalists, research stated, "Recently it was shown that monoterpenes in tomato trichomes (Solarium lycopersicum) are synthesized by phellandrene synthase 1 (PHS1) from the non-canonical substrate neryl diphosphate (NPP), the cis-isomer of geranyl diphosphate (GPP). As PHS1 accepts both NPP and GPP substrates forming different monoterpenes, it was overexpressed in tomato fruits to test if NPP is also available in a tissue highly active in carotenoid production."
The news correspondents obtained a quote from the research from Purdue University, "However, transgenic fruits overexpressing PHS1 produced only small amounts of GPP-derived PHS1 monoterpene products, indicating the absence of endogenous NPP. Therefore, NPP formation was achieved by diverting the metabolic flux from carotenoids via expression of tomato neryl diphosphate synthase 1 (NDPS1). NDPS1 transgenic fruits produced NPP-derived monoterpenes, including nerol, neral and geranial, while displaying reduced lycopene content. NDPS1 co-expression with PHS1 resulted in a monoterpene blend, including beta-phellandrene, similar to that produced from NPP by PHS1 in vitro and in trichomes. Unexpectedly, PHS1 x NDPS1 fruits showed recovery of lycopene levels compared to NDPS1 fruits, suggesting that redirection of metabolic flux is only partially responsible for the reduction in carotenoids. In vitro assays demonstrated that NPP serves as an inhibitor of geranylgeranyl diphosphate synthase, thus its consumption by PHS1 leads to recovery of lycopene levels. Monoterpenes produced in PHS1 x NDPS1 fruits contributed to direct plant defense negatively affecting feeding behavior of the herbivore Helicoverpa zea and displaying antifungal activity against Botrytis cinerea."
According to the news reporters, the research concluded: "These results show that NPP-derived terpenoids can be produced in plant tissues; however, NPP has to be consumed to avoid negative impacts on plant metabolism."
For more information on this research see: Metabolic engineering of monoterpene biosynthesis in tomato fruits via introduction of the non-canonical substrate neryl diphosphate. Metabolic Engineering, 2014;24():107-116. Metabolic Engineering can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Metabolic Engineering - www.elsevier.com/wps/product/cws_home/622913)
Our news journalists report that additional information may be obtained by contacting M. Gutensohn, Purdue University, Dept. of Biochem, West Lafayette, IN 47907, United States. Additional authors for this research include T.T.H. Nguyen, R.D. McMahon, I. Kaplan, E. Pichersky and N. Dudareva (see also Polyphosphates).
Keywords for this news article include: Anions, Indiana, Synthase, Carotenoids, Diphosphates, Electrolytes, Monoterpenes, United States, West Lafayette, Polyphosphates, Phosphoric Acids, Biological Pigments, Phosphorus Compounds, Enzymes and Coenzymes, Metabolic Engineering, North and Central America
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