Studies from Fudan University Further Understanding of Cytoplasmic Vesicles (Scavenger receptor-recognized and enzyme-responsive nanoprobe for fluorescent labeling of lysosomes in live cells)
By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Cytoplasmic Vesicles have been published. According to news originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Lysosomal imaging represents a potent tool for investigating the organization of related cellular events and their modulation via diagnostic and therapeutic approaches. However, specific labeling of the lysosome in live cells is a significant challenge."
Our news journalists obtained a quote from the research from Fudan University, "Taking advantage of the inherent lysosomal entry of nanoparticles and unique digestive inclusions in the lysosome, we developed a nanoparticle-based, enzyme-switchable fluorescence OFF-ON strategy for specific labeling of the lysosome and further imaging of extracellular acidification-induced lysosome trafficking in living cells. The nanoprobe comprised a 16 nm spherical gold nanoparticle as the core and an enzyme-responsive oligomer of fluorescein-conjugated oligo(4-vinyl-phenyl phosphate) as the shell. Due to quenching of the core gold nanoparticle, the nanoprobe was non-fluorescent. After incubation with cancer cells, the nanoprobe was rapidly internalized via scavenger receptor-mediated endocytosis and significantly shuffled into the lysosome. The nanoprobe specifically lighted up the lysosome owing to lysosome-induced fluorescence enhancement. Specifically, digestive inclusions in the lysosome hydrolyzed and released gold-quenched fluorescein molecules, leading to significant augmentation of fluorescence. On account of specific lysosomal labeling, the nanoprobe effectively facilitated imaging of a 4-6 gm anterograde trafficking event of the lysosome from the perinuclear region to the cell surface when an acidic extracellular environment developed."
According to the news editors, the research concluded: "Our findings collectively highlight the use of nanoprobes for lysosomal imaging."
For more information on this research see: Scavenger receptor-recognized and enzyme-responsive nanoprobe for fluorescent labeling of lysosomes in live cells. Biomaterials, 2014;35(27):7870-7880. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news correspondents report that additional information may be obtained from Y.B. Fan, Fudan University, Inst Biomed Sci, Dept. of Chem, State Key Lab Mol Engn Polymers, Shanghai 200433, People's Republic of China. Additional authors for this research include F.Y. Li and D.Y. Chen (see also Cytoplasmic Vesicles).
Keywords for this news article include: Asia, Shanghai, Lysosomes, LDL Receptors, Nanotechnology, Membrane Proteins, Gold Nanoparticles, Intracellular Space, Scavenger Receptors, Cytoplasmic Vesicles, Emerging Technologies, Enzymes and Coenzymes, Immunologic Receptors, Cytoplasmic Structures, People's Republic of China
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