Studies Conducted at Huazhong University of Science and Technology on Stem Cells Recently Reported [shRNA targeting Bmi-1 sensitizes CD44(+) nasopharyngeal cancer stem-like cells to radiotherapy]
By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Stem Cell Research have been published. According to news reporting originating in Hubei, People's Republic of China, by NewsRx journalists, research stated, "Accumulating evidence indicates that cancer stem cells (CSCs) are involved in resistance to radiation therapy (RT). Bmi-1, a member of the Polycomb family of transcriptional repressors, is essential for maintaining the self-renewal abilities of stem cells and overexpression of Bmi-1 correlates with cancer therapy failure."
The news reporters obtained a quote from the research from the Huazhong University of Science and Technology, "Our previous study identified that the CD44(+) nasopharyngeal cancer (NPC) cells may be assumed as one of markers of nasopharyngeal carcinoma cancer stem cell-like cells (CSC-LCs) and Bmi-1 is overexpressed in CD44(+) NPC. In the present study, we used RNA interference technology to knock down the expression of Bmi-1 in CD44+ NPC cells, and then measured the radiation response by clonogenic cell survival assay. DNA repair was monitored by gamma H2AX foci formation. Bmi-1 downstream relative gene and protein expression of p16, p14, p53 were assessed by western blotting and real-time PCR. Cell cycle and apoptosis were detected by flow cytometry assays. We found that Bmi-1 knockdown prolonged G1 and enhanced the radiation-induced G2/M arrest, inhibited DNA damage repair, elevated protein p16, p14 and p53 expression, leading to increased apoptosis in the radiated CD44(+) cells. These data suggest that Bmi-1 downregulation increases the radiosensitivity to CD44(+) NPC CSC-LCs."
According to the news reporters, the research concluded: "Bmi-1 is a potential target for increasing the sensitivity of NPC CSCs to radiotherapy."
For more information on this research see: shRNA targeting Bmi-1 sensitizes CD44(+) nasopharyngeal cancer stem-like cells to radiotherapy. Oncology Reports, 2014;32(2):764-770. Oncology Reports can be contacted at: Spandidos Publ Ltd, Pob 18179, Athens, 116 10, Greece (see also Stem Cell Research).
Our news correspondents report that additional information may be obtained by contacting X.H. Xu, Huazhong University of Science & Technology, Tongji Med College, Canc Center, Union Hosp, Wuhan 430074, Hubei, People's Republic of China. Additional authors for this research include X.Y. Liu, J. Su, D.J. Li, Q. Huang, M.Q. Lu, F. Yi, J.H. Ren and W.H. Chen.
Keywords for this news article include: Asia, Hubei, Cancer, Genetics, Oncology, p53 Gene, Radiotherapy, Stem Cell Research, People's Republic of China
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