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Researchers' Work from University of Oxford Focuses on Nanotechnology (Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact...

August 19, 2014



Researchers' Work from University of Oxford Focuses on Nanotechnology (Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions)

By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Nanotechnology is now available. According to news reporting originating from Oxford, United Kingdom, by NewsRx correspondents, research stated, "We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K)."

Our news editors obtained a quote from the research from the University of Oxford, "Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm only. We demonstrate that the single molecule characteristics of reversible photobleaching of mEGFP and mVenus at liquid nitrogen temperature are suitable for the basic concept of single molecule localization microscopy."

According to the news editors, the research concluded: "This enabled us to perform super-resolution imaging of vitrified biological samples and to visualize structures in unperturbed fast frozen cells for the first time with a structural resolution of similar to 125 nm (average single molecule localization accuracy similar to 40 nm), corresponding to a 3-5 fold resolution improvement."

For more information on this research see: Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions. Nano Letters, 2014;14(7):4171-4175. Nano Letters can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Nano Letters - www.pubs.acs.org/journal/nalefd)

The news editors report that additional information may be obtained by contacting R. Kaufmann, University of Oxford, Dept. of Biochem, Oxford OX1 3QU, United Kingdom. Additional authors for this research include P. Schellenberger, E. Seiradake, I.M. Dobbie, E.Y. Jones, I. Davis, C. Hagen and K. Grunewald (see also Nanotechnology).

Keywords for this news article include: Oxford, Europe, United Kingdom, Nanotechnology

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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