News Column

Researchers from University of Louisiana Report Findings in Scleroproteins (Fish gelatin thin film standards for biological application of PIXE)

August 19, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Proteins is now available. According to news reporting out of Monroe, Louisiana, by NewsRx editors, research stated, "There exists a critical need to understand the flow and accumulation of metallic ions, both naturally occurring and those introduced to biological systems. In this paper the results of fabricating thin film elemental biological standards containing nearly any combination of trace elements in a protein matrix are presented."

Our news journalists obtained a quote from the research from the University of Louisiana, "Because it is capable of high elemental sensitivity, particle induced X-ray emission spectrometry (PIXE) is an excellent candidate for in situ analysis of biological tissues. Additionally, the utilization of microbeam PIXE allows the determination of elemental concentrations in and around biological cells. However, obtaining elemental reference standards with the same matrix constituents as brain tissue is difficult. An excellent choice for simulating brain-like tissue is Norland photoengraving glue which is derived from fish skin. Fish glue is water soluble, liquid at room temperature, and resistant to dilute acid. It can also be formed into a thin membrane which dries into a durable, self-supporting film. Elements of interest are introduced to the fish glue in precise volumetric additions of well quantified atomic absorption standard solutions. In this study GeoPIXE analysis package is used to quantify elements intrinsic to the fish glue as well as trace amounts of manganese added to the sample. Elastic (non-Rutherford) backscattered spectroscopy (EBS) and the 1.734 MeV proton-on-carbon C-12(p,p)C-12 resonance is used for a normalization scheme of the PIXE spectra to account for any discrepancies in X-ray production arising from thickness variation of the prepared standards."

According to the news editors, the research concluded: "It is demonstrated that greater additions of the atomic absorption standard cause a viscosity reduction of the liquid fish glue resulting in thinner films but the film thickness can be monitored by using simultaneous PIXE and EBS proton data acquisition."

For more information on this research see: Fish gelatin thin film standards for biological application of PIXE. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 2014;332():37-41. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Proteins).

Our news journalists report that additional information may be obtained by contacting J.E. Manuel, Univ Louisiana Monroe, Dept. of Basic Pharmaceut Sci, Monroe, LA 71209, United States. Additional authors for this research include B. Rout, S.Z. Szilasi, G. Bohara, J. Deaton, H. Luyombya, K.P. Briski and G.A. Glass.

Keywords for this news article include: Monroe, Gelatin, Louisiana, United States, Scleroproteins, North and Central America

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Source: Life Science Weekly

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