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Researchers from University of Halle-Wittenberg Report New Studies and Findings in the Area of Enzyme Inhibitors (Articular cartilage chondrocytes...

August 20, 2014



Researchers from University of Halle-Wittenberg Report New Studies and Findings in the Area of Enzyme Inhibitors (Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism)

By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Biotechnology are discussed in a new report. According to news originating from Halle, Germany, by NewsRx correspondents, research stated, "Sex hormones, especially estrogens, have been implicated in articular cartilage metabolism and the pathogenesis of postmenopausal osteoarthritis. The conversion by aromatase (CYP19A1) of androstenedione into estrone (E1) and of testosterone into 17 beta-estradiol (E2) plays a key role in the endogenous synthesis of estrogens in tissue."

Our news journalists obtained a quote from the research from the University of Halle-Wittenberg, "We analyzed the expression of aromatase (CYP19A1) in immortalized C-28/I2 and T/C-28a2 chondrocytes, as well as in cultured primary human articular chondrocytes and human articular cartilage tissue, by means of RT-PCR, Western blotting and immunohistochemistry. By means of quantitative RT-PCR and enzyme-linked immunosorbent assay, we also determined whether the aromatase inhibitor letrozole influences estrogen metabolism of cultured chondrocytes in immortalized C-28/I2 chondrocytes. Aromatase mRNA was detected in both immortalized chondrocyte cell lines, in cultured primary human chondrocytes, and in human articular cartilage tissue. By means of Western blot analysis, aromatase was detected at the protein level in articular cartilage taken from various patients of both sexes and different ages. Cultured primary human articular chondrocytes, C-28/I2 and T/C-28a2, and human articular cartilage tissue reacted with antibodies for aromatase. Incubation of C-28/I2 chondrocytes with 10(-11) M to 10(-7) M letrozole as an aromatase inhibitor revealed significantly increased amounts of the mRNAs of the enzyme cytochrome P4501A1 (CYP1A1), which is involved in the catagen estrogen metabolism, and of the estrogen receptors ER-alpha and ER-beta. Concomitantly, synthesis of estrone (E1) was significantly downregulated after incubation with letrozole. We demonstrate that human articular cartilage expresses aromatase at the mRNA and protein levels. Blocking of estrone synthesis by the aromatase inhibitor letrozole is counteracted by an increase in ER-alpha and ER-beta. In addition, CYP1A1, an enzyme involved in catabolic estrogen metabolism, is upregulated. This suggests that articular chondrocytes use ERs functionally."

According to the news editors, the research concluded: "The role of endogenous synthesized estrogens in articular cartilage health remains to be elucidated."

For more information on this research see: Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism. Arthritis Research & Therapy, 2014;16(2):450-458. Arthritis Research & Therapy can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; Arthritis Research & Therapy - arthritis-research.com/)

The news correspondents report that additional information may be obtained from M. Schicht, University of Halle Wittenberg, Univ Hosp Orthoped & Phys Med, D-06097 Halle, Saale, Germany. Additional authors for this research include J. Ernst, A. Nielitz, L. Fester, M. Tsokos, S.S. Guddat, L. Brauer, J. Bechmann, K.S. Delank, D. Wohlrab, F. Paulsen and H. Claassen (see also Biotechnology).

Keywords for this news article include: Biotechnology, Halle, Europe, Germany, Estrone, Cytochromes, Chondrocytes, Hemeproteins, 17-Ketosteroids, Oxidoreductases, Gonadal Hormones, Enzyme Inhibitors, Estradiol Congeners, Aromatase Inhibitors, Steroid Hydroxylases, Enzymes and Coenzymes, Connective Tissue Cells, Mixed Function Oxygenases

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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