Researchers from National Tsing Hua University Report New Studies and Findings in the Area of Immunoglobulins (Fabrication of Antibody Microarrays by Light-Induced Covalent and Oriented Immobilization)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Immunology are presented in a new report. According to news reporting originating in Hsinchu, Taiwan, by NewsRx journalists, research stated, "Antibody microarrays have important applications for the sensitive detection of biologically important target molecules and as biosensors for clinical applications. Microarrays produced by oriented immobilization of antibodies generally have higher antigen-binding capacities than those in which antibodies are immobilized with random orientations."
The news reporters obtained a quote from the research from National Tsing Hua University, "Here, we present a UV photo-cross-linking approach that utilizes boronic acid to achieve oriented immobilization of an antibody on a surface while retaining the antigen-binding activity of the immobilized antibody. A photoactive boronic acid probe was designed and synthesized in which boronic acid provided good affinity and specificity for the recognition of glycan chains on the Fc region of the antibody, enabling covalent tethering to the antibody upon exposure to UV light. Once irradiated with optimal UV exposure (16 mW/cm(2)), significant antibody immobilization on a boronic acid-presenting surface with maximal antigen detection sensitivity in a single step was achieved, thus obviating the necessity of prior antibody modifications. The developed approach is highly modular, as demonstrated by its implementation in sensitive sandwich immunoassays for the protein analytes Ricinus communis agglutinin 120, human prostate-specific antigen, and interleukin-6 with limits of detection of 7.4, 29, and 16 pM, respectively. Furthermore, the present system enabled the detection of multiple analytes in samples without any noticeable cross-reactivities."
According to the news reporters, the research concluded: "Antibody coupling via the use of boronic acid and UV light represents a practical, oriented immobilization method with significant implications for the construction of a large array of immunosensors for diagnostic applications."
For more information on this research see: Fabrication of Antibody Microarrays by Light-Induced Covalent and Oriented Immobilization. ACS Applied Materials & Interfaces, 2014;6(13):10452-10460. ACS Applied Materials & Interfaces can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Applied Materials & Interfaces - www.pubs.acs.org/journal/aamick)
Our news correspondents report that additional information may be obtained by contacting A.K. Adak, Natl Tsing Hua Univ, Dept. of Chem, Hsinchu 300, Taiwan. Additional authors for this research include B.Y. Li, L.D. Huang, T.W. Lin, T.C. Chang, K.C. Hwang and C.C. Lin (see also Immunology).
Keywords for this news article include: Asia, Antibodies, Taiwan, Hsinchu, Immunology, Boronic Acids, Blood Proteins, Boron Compounds, Immunoglobulins, Noncarboxylic Acids
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