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Researchers from Imperial College London Report Details of New Studies and Findings in the Area of Biophotonics (Automated fluorescence lifetime...

August 20, 2014



Researchers from Imperial College London Report Details of New Studies and Findings in the Area of Biophotonics (Automated fluorescence lifetime imaging plate reader and its application to Forster resonant energy transfer readout of Gag protein ...)

By a News Reporter-Staff News Editor at Biotech Week -- A new study on Biotechnology is now available. According to news reporting from London, United Kingdom, by NewsRx journalists, research stated, "Fluorescence lifetime measurements can provide quantitative readouts of local fluorophore environment and can be applied to biomolecular interactions via Forster resonant energy transfer (FRET). Fluorescence lifetime imaging (FLIM) can therefore provide a high content analysis (HCA) modality to map protein-protein interactions (PPIs) with applications in drug discovery, systems biology and basic research."

The news correspondents obtained a quote from the research from Imperial College London, "We present here an automated multiwell plate reader able to perform rapid unsupervised optically sectioned FLIM of fixed and live biological samples and illustrate its potential to assay PPIs through application to Gag protein aggregation during the HIV life cycle. We demonstrate both hetero-FRET and homo-FRET readouts of protein aggregation and report the first quantitative evaluation of a FLIM HCA assay by generating dose response curves through addition of an inhibitor of Gag myristoylation."

According to the news reporters, the research concluded: "Z' factors exceeding 0.6 are realised for this FLIM FRET assay."

For more information on this research see: Automated fluorescence lifetime imaging plate reader and its application to Forster resonant energy transfer readout of Gag protein aggregation. Journal of Biophotonics, 2013;6(5):398-408. (Wiley-Blackwell - www.wiley.com/; Journal of Biophotonics - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648)

Our news journalists report that additional information may be obtained by contacting D. Alibhai, Institute of Chemical Biology, Dept. of Chemistry, Imperial College London, South Kensington Campus, London, UK. Additional authors for this research include D.J. Kelly, S. Warren, S. Kumar, A. Margineau, R.A. Serwa, E. Thinon, Y. Alexandrov, E.J. Murray, F. Stuhmeier, E.W. Tate, M.A. Neil, C. Dunsby and P.M French (see also Biotechnology).

Keywords for this news article include: Biotechnology, London, Europe, United Kingdom.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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