Reports Summarize Enzymes and Coenzymes Study Results from Delft University of Technology (Enzymatic hydration activity assessed by selective spectrophotometric detection of alcohols: a novel screening assay using oleate hydratase as a model ...)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting out of Delft, Netherlands, by NewsRx editors, research stated, "Hydroxy fatty acids (HFAs) are high-added-value compounds, which are incorporated in polymers, lubricants, emulsifiers and stabilizers and have potential medicinal use. In nature, HFAs are regio-specifically synthesized by several enzymes, including P450 monooxygenases, lipoxygenases, hydratases, 12-hydroxylases, and diol synthases."
Our news journalists obtained a quote from the research from the Delft University of Technology, "The growing demand for HFAs warrants the development of simple and efficient analytical methods that enable high-throughput detection of the hydroxylated product in the presence of its unsaturated precursor. Herein a novel high-throughput assay for the detection of alcohols is described using oleate hydratase (OHase, EC 18.104.22.168) from Elizabethkingia meningoseptica as the model enzyme. The developed assay is based on the selective spectrophotometric detection of alkyl nitrites formed upon the reaction between the hydroxyl group and nitrous acid. The assay proved to discriminate between unsaturated fatty acids as well as small cyclic and acyclic unsaturated alkenes and their corresponding alcohols. Lower detection limits were 1.5-3 mM with excellent Z'-factors. Enzymatic reactions using OHase with oleic acid resulted in somewhat lower Z-factors for various enzyme preparations."
According to the news editors, the research concluded: "This small scale assay can enable fast discovery of new microorganisms or improved enzymes from mutant libraries and will be useful for biocatalytic strategies involving fatty acid (de)hydrating enzymes."
For more information on this research see: Enzymatic hydration activity assessed by selective spectrophotometric detection of alcohols: a novel screening assay using oleate hydratase as a model enzyme. Biotechnology Journal, 2014;9(6):814-21. (Wiley-Blackwell - www.wiley.com/; Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314)
Our news journalists report that additional information may be obtained by contacting A. Hiseni, Biocatalysis and Organic Chemistry, Dept. of Biotechnology, Delft University of Technology, Delft, Netherlands. Additional authors for this research include R. Medici, I.W. Arends and L.G Otten (see also Enzymes and Coenzymes).
Keywords for this news article include: Delft, Europe, Alcohols, Netherlands, Organic Chemicals, Enzymes and Coenzymes.
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