Reports on Small Interference RNAs (siRNAs) from China Pharmaceutical University Provide New Insights (PEGylated carboxymethyl chitosan/calcium phosphate hybrid anionic nanoparticles mediated hTERT siRNA delivery for anticancer therapy)
By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Small Interference RNAs (siRNAs) have been published. According to news reporting originating in Jiangsu, People's Republic of China, by NewsRx journalists, research stated, "Lack of safe and effective delivery vehicle is the main obstacle for siRNA mediated cancer therapy. In this study, we synthesized a pH-sensitive polymer of PEG grafted carboxymethyl chitosan (PEG-CMCS) and developed anionic-charged hybrid nanoparticles of PEG-CMCS and calcium phosphate (Cap) for siRNA delivery through a single-step self-assembly method in aqueous condition."
The news reporters obtained a quote from the research from China Pharmaceutical University, "The formed nanoparticles with charge of around -8.25 mv and average diameter of 102.1 nm exhibited efficient siRNA encapsulation and enhanced colloidal and serum stability. The test in vitro indicated that the nanoparticles entered into HepG2 cells by endocytosis, and achieved endosomal escape of siRNA effectively due to the pH-responsive disassembly of nanoparticles and dissolution of CaP in the endosome. Reporter gene silencing assay showed that luciferase siRNA delivered by the anionic nanoparticles could achieve gene silencing efficacy comparable to that of conventional Lipofectamine 2000. Additionally, dramatic hTERT knockdown mediated by the anionic nanoparticles transfection induced significant apoptosis of HepG2 cells in vitro. After intravenous injection in tumor-bearing BALB/c nude mice, the nanopartides specifically accumulated into tumor regions by EPR effect, leading to efficient and specific gene silencing sequentially. Most importantly, the nanoparticles carrying hTERT siRNA inhibited tumor growth significantly via silencing hTERT expression and inducing cells apoptosis in HepG2 tumor xenograft. Moreover, comprehensive safety studies of the nanoparticles confirmed their superior safety both in vitro and in vivo."
According to the news reporters, the research concluded: "We concluded that the PEG-CMCS/CaP hybrid anionic nanoparticles possessed potential as a safe and effective siRNA delivery system for anticancer therapy."
For more information on this research see: PEGylated carboxymethyl chitosan/calcium phosphate hybrid anionic nanoparticles mediated hTERT siRNA delivery for anticancer therapy. Biomaterials, 2014;35(27):7978-7991. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
Our news correspondents report that additional information may be obtained by contacting Y. Xie, China Pharmaceutical University, Dept. of Pharmaceut, State Key Lab Nat Med, Nanjing 210009, Jiangsu, People's Republic of China. Additional authors for this research include H.Z. Qiao, Z.G. Su, M.L. Chen, Q.N. Ping and M.J. Sun (see also Small Interference RNAs (siRNAs)).
Keywords for this news article include: Asia, Anions, Jiangsu, Therapy, Genetics, Nanoparticle, Nanotechnology, Phosphoric Acids, Calcium Compounds, Calcium Phosphates, Inorganic Chemicals, Phosphorus Compounds, Emerging Technologies, People's Republic of China, Small Interference RNAs (siRNAs)
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