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Reports from State University of Campinas Provide New Insights into Haemophilus influenzae (Haemophilus influenzae porine ompP2 gene transfer...

August 22, 2014



Reports from State University of Campinas Provide New Insights into Haemophilus influenzae (Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence ...)

By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- New research on Proteobacteria is the subject of a report. According to news reporting from Sao Paulo, Brazil, by NewsRx journalists, research stated, "H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup -aegyptius -as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor."

The news correspondents obtained a quote from the research from the State University of Campinas, "This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased. In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains. The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type."

According to the news reporters, the research concluded: "Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines."

For more information on this research see: Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity. Journal of Nanobiotechnology, 2014;12():14. (BioMed Central - www.biomedcentral.com/; Journal of Nanobiotechnology - www.jnanobiotechnology.com)

Our news journalists report that additional information may be obtained by contacting J.N. Varela, LABIOTEC - Biotechnology Laboratory, Dept. of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, Sao Paulo, Brazil. Additional authors for this research include M.C. Amstalden, R.F. Pereira, L.M. de Hollanda, H.J. Ceragioli, V. Baranauskas and M. Lancellotti (see also Proteobacteria).

Keywords for this news article include: Brazil, Genetics, Sao Paulo, South America, Pasteurellaceae, Gammaproteobacteria, Gram Negative Bacteria, Haemophilus influenzae, Gram Negative Facultatively Anaerobic Rods.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Genomics & Genetics Weekly


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