Reports from National University Describe Recent Advances in Zinc Compounds (Zinc oxide nanoparticles based microfluidic immunosensor applied in congenital hypothyroidism screening)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Zinc Compounds. According to news reporting originating from San Luis, Argentina, by NewsRx correspondents, research stated, "In this article, we present an innovative approach for congenital hypothyroidism (CHT) screening. This pathology is the most common preventable cause of mental retardation, affecting newborns around the world."
Our news editors obtained a quote from the research from National University, "Its consequences could be avoided with an early diagnosis through the thyrotropin (TSH) level measurement. To accomplish the determination of TSH, synthesized zinc oxide (ZnO) nanobeads (NBs) covered by chitosan (CH), ZnO-CH NBs, were covalently attached to the central channel of the designed microfluidic device. These beads were employed as platform for anti-TSH monoclonal antibody immobilization to specifically recognize and capture TSH in neonatal samples without any special pretreatment. Afterwards, the amount of this trapped hormone was quantified by horseradish peroxidase (HRP)-conjugated anti-TSH antibody. HRP reacted with its enzymatic substrate in a redox process, which resulted in the appearance of a current whose magnitude was directly proportional to the level of TSH in the neonatal sample. The structure and morphology of synthesized ZnO-CH NBs were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The calculated detection limits for electrochemical detection and the enzyme-linked immunosorbent assay procedure were 0.00087 mu UI mL(-1) and 0.015 mu UI mL(-1), respectively, and the within- and between-assay coefficients of variation were below 6.31 % for the proposed method. According to the cut-off value for TSH neonatal screening, a reasonably good limit of detection was achieved."
According to the news editors, the research concluded: "These above-mentioned features make the system advantageous for routine clinical analysis adaptation."
For more information on this research see: Zinc oxide nanoparticles based microfluidic immunosensor applied in congenital hypothyroidism screening. Analytical and Bioanalytical Chemistry, 2014;406(19):4677-4684. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
The news editors report that additional information may be obtained by contacting M.A. Seia, Natl Univ San Luis, INQUISAL, Dept. of Chem, CONICET, San Luis, Argentina. Additional authors for this research include S.V. Pereira, M.A. Fernandez-Baldo, I.E. De Vito, J. Raba and G.A. Messina (see also Zinc Compounds).
Keywords for this news article include: San Luis, Argentina, Chemicals, Chemistry, Zinc Oxide, Nanoparticle, South America, Nanotechnology, Zinc Compounds, Emerging Technologies
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