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Recent Studies from Baylor University College of Medicine Add New Data to Nucleic Acids Research (A piggyBac-based reporter system for scalable in...

August 19, 2014



Recent Studies from Baylor University College of Medicine Add New Data to Nucleic Acids Research (A piggyBac-based reporter system for scalable in vitro and in vivo analysis of 3 ' untranslated region-mediated gene regulation)

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Life Science Research is the subject of a report. According to news reporting originating in Houston, Texas, by NewsRx journalists, research stated, "Regulation of messenger ribonucleic acid (mRNA) subcellular localization, stability and translation is a central aspect of gene expression. Much of this control is mediated via recognition of mRNA 3' untranslated regions (UTRs) by microRNAs (miRNAs) and RNA-binding proteins."

The news reporters obtained a quote from the research from the Baylor University College of Medicine, "The gold standard approach to assess the regulation imparted by a transcript's 3' UTR is to fuse the UTR to a reporter coding sequence and assess the relative expression of this reporter as compared to a control. Yet, transient transfection approaches or the use of highly active viral promoter elements may overwhelm a cell's post-transcriptional regulatory machinery in this context. To circumvent this issue, we have developed and validated a novel, scalable piggyBac-based vector for analysis of 3' UTR-mediated regulation in vitro and in vivo. The vector delivers three independent transcription units to the target genome-a selection cassette, a turboGFP control reporter and an experimental reporter expressed under the control of a 3' UTR of interest. The pBUTR (piggyBac-based 3' Un Translated Region reporter) vector performs robustly as a siRNA/miRNA sensor, in established in vitro models of post-transcriptional regulation, and in both arrayed and pooled screening approaches."

According to the news reporters, the research concluded: "The vector is robustly expressed as a transgene during murine embryogenesis, highlighting its potential usefulness for revealing post-transcriptional regulation in an in vivo setting."

For more information on this research see: A piggyBac-based reporter system for scalable in vitro and in vivo analysis of 3 ' untranslated region-mediated gene regulation. Nucleic Acids Research, 2014;42(10):48-61. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)

Our news correspondents report that additional information may be obtained by contacting A. Chaudhury, Baylor College of Medicine, Dan L Duncan Canc Center, Houston, TX 77030, United States. Additional authors for this research include N. Kongchan, J.P. Gengler, V. Mohanty, A.E. Christiansen, J.M. Fachini, J.F. Martin and J.R. Neilson (see also Life Science Research).

Keywords for this news article include: Texas, Houston, United States, Life Science Research, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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