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Recent Findings in Immunoglobulins Described by Researchers from University of Alberta (Applications of low-intensity pulsed ultrasound to increase...

August 19, 2014



Recent Findings in Immunoglobulins Described by Researchers from University of Alberta (Applications of low-intensity pulsed ultrasound to increase monoclonal antibody production in CHO cells using shake flasks or wavebags)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Immunology. According to news reporting out of Edmonton, Canada, by NewsRx editors, research stated, "Many technologies, such as cell line screening and host cell engineering, culture media optimization and bioprocess optimization, have been proposed to increase monoclonal antibody (mAb) production in Chinese Hamster Ovary (CHO) cells. Unlike the existing biochemical approaches, we investigated stimulation using low-intensity pulsed ultrasound (LIPUS) as a purely physical approach, offering enhanced scalability, contamination control and cost-efficiency, while demonstrating significantly increased cell growth and antibody production."

Our news journalists obtained a quote from the research from the University of Alberta, "It was found that daily ultrasound treatments at 40 mW/cm(2) for 5 min during cell culture increased the production of human anti-IL-8 antibody by more than 30% using 10 or 30 mL shake flasks. Further increasing the ultrasound dosage (either intensities or the treatment duration) did not appreciably increase cell growth or antibody production, however feeding the culture with additional highly-concentrated nutrients, glucose and amino acids (glutamine in this case), did further increase cell growth and antibody titer to 35%. Similar ultrasound treatments (40 mW/cm(2), 5 min per day) when scaled up to larger volume wavebags, resulted in a 25% increase in antibody production. Increased antibody production can be attributed to both elevated cell count and the ultrasound stimulation. Theoretical study of underlying mechanisms was performed through the simulations of molecular dynamics using the AMBER software package, with results showing that LIPUS increases cell permeability. The significance of this study is that LIPUS, as a physical-based stimulation approach, can be externally applied to the cell culture without worrying about contamination. By combining with the existing technologies in antibody production, LIPUS can achieve additional mAb yields."

According to the news editors, the research concluded: "Because it can be easily integrated with existing cell culture apparatuses, the technology is expected to be more acceptable by the end users."

For more information on this research see: Applications of low-intensity pulsed ultrasound to increase monoclonal antibody production in CHO cells using shake flasks or wavebags. Ultrasonics, 2014;54(6):1439-47. (Elsevier - www.elsevier.com; Ultrasonics - www.elsevier.com/wps/product/cws_home/525452)

Our news journalists report that additional information may be obtained by contacting Y. Zhao, Dept. of Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada. Additional authors for this research include J. Xing, J.Z. Xing, W.T. Ang and J. Chen (see also Immunology).

Keywords for this news article include: Antibodies, Canada, Alberta, Edmonton, CHO Cells, Cell Line, Immunology, Technology, Blood Proteins, Immunoglobulins, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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