New DNA Research Study Results from Nanjing University Described (Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on DNA Research. According to news originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "Contamination of heavy metal ions in an aquatic environment poses a serious threat to human health. More seriously, heavy metal ions are usually present in the environment in a mixture, and the synergetic toxicity of multiple heavy metal ions is revealed (Aragay et al. Chem. Rev. 2011, 111, 3433; Chu et al. Aquat. Toxicol. 2002, 61, 53)."
Our news journalists obtained a quote from the research from Nanjing University, "Unfortunately, most of the existing methods based on DNA sequences are focusing on the detection of one type of metal ions. Simple and multiplexed detection of multiple metal ions has been poorly investigated and remains challenging. Here, we re-engineered the DNA sequences for Pb2+, Hg2+, and Ag+, through which the binding of multiple metal ions initiated the self-assembly of these DNA sequences. On the basis of our rationally designed multicolor fluorescent labeling of the DNA sequences, cascade fluorescence resonance energy transfer (FRET) occurred. As a result, a fingerprint fluorescent spectrum was produced to indicate the presence of a single type of metal ions or multiple metal ions."
According to the news editors, the research concluded: "The major advantages of our cascade FRET fingerprint technology include the following: (1) the 'mix and read' detection mode in homogeneous solution is simple without the need of complicated instruments; (2) only single excitation is required to provide the cascade FRET fingerprint spectrum; (3) multiplexed detection capability can be realized intuitively and sensitively."
For more information on this research see: Metal Ion-Mediated Assembly of DNA Nanostructures for Cascade Fluorescence Resonance Energy Transfer-Based Fingerprint Analysis. Analytical Chemistry, 2014;86(14):7084-7087. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from J.Y. Xia, Nanjing Univ Posts & Telecommun, IAM, Nanjing, Jiangsu, People's Republic of China. Additional authors for this research include M.H. Lin, X.L. Zuo, S. Su, L.H. Wang, W. Huang, C.H. Fan and Q. Huang (see also DNA Research).
Keywords for this news article include: Asia, Jiangsu, DNA Research, Nanostructural, Nanostructures, Nanotechnology, Emerging Technologies, People's Republic of China
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