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New Data from University of Jena Illuminate Findings in Surface Plasmon Resonance (A Novel Method for the Assessment of Targeted PEI-Based...

August 21, 2014



New Data from University of Jena Illuminate Findings in Surface Plasmon Resonance (A Novel Method for the Assessment of Targeted PEI-Based Nanoparticle Binding Based on a Static Surface Plasmon Resonance System)

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Surface Plasmon Resonance. According to news reporting originating in Jena, Germany, by NewsRx journalists, research stated, "The delivery of nucleic acids is a major hurdle in gene therapy or therapeutic gene knockdown, and the development of intelligent and safe nanoparticles as carrier systems is thus under intense investigation. The introduction of ligands for their targeted delivery is of major interest."

The news reporters obtained a quote from the research from the University of Jena, "Here, we describe a novel approach for the analysis of the binding properties of antibody-functionalized nanoparticles, using surface plasmon resonance (SPR) in a static cuvette system. By chemical coupling of the Epidermal Growth Factor Receptor (EGFR)-specific antibody cetuximab to poly(ethylene imine) (PEI) via a PEG-spacer and subsequent DNA or siRNA complexation, we generated targeted nanoplexes with low surface charge. Antibody-mediated uptake into EGFR overexpressing cells was observed. SPR measurements with use of a novel, protein A-based sandwich system for the immobilization of the target receptor in its correct steric orientation allowed the analysis of the specific PEI-PEG-cetuximab binding to EGFR and the determination of binding affinities. Importantly, our cuvette-based SPR assay system was also suitable for the monitoring of ligand-mediated nanoparticle binding, without convection or shear stress."

According to the news reporters, the research concluded: "We conclude that our SPR sandwich system allows the precise analysis of the binding of ligand-functionalized nanoparticles in real-time, and we thus establish SPR for the in vitro evaluation of ligand modifications for generating targeted nanoparticles."

For more information on this research see: A Novel Method for the Assessment of Targeted PEI-Based Nanoparticle Binding Based on a Static Surface Plasmon Resonance System. Analytical Chemistry, 2014;86(14):6827-6835. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news correspondents report that additional information may be obtained by contacting S. Hobel, University of Jena, Dept. of Pharmaceut Technol, Fac Biol & Pharm, Inst Pharm, D-07745 Jena, Germany. Additional authors for this research include D. Vornicescu, M. Bauer, D. Fischer, M. Keusgen and A. Aigner (see also Surface Plasmon Resonance).

Keywords for this news article include: Jena, Antibodies, Europe, Germany, Immunology, Nanoparticle, Blood Proteins, Nanotechnology, Immunoglobulins, Emerging Technologies, Surface Plasmon Resonance

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gene Therapy Weekly


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