New Biophotonics Findings from Bar-Ilan University Discussed [The influence of dead time related distortions on live cell fluorescence lifetime imaging (FLIM) experiments]
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting originating from Ramat Gan, Israel, by NewsRx correspondents, research stated, "Recent developments in the field of fluorescence lifetime imaging microscopy (FLIM) techniques allow the use of high repetition rate light sources in live cell experiments. For light sources with a repetition rate of 20-100 MHz, the time-correlated single photon counting (TCSPC) FLIM systems suffer serious dead time related distortions, known as 'inter-pulse pile-up'."
Our news editors obtained a quote from the research from Bar-Ilan University, "The objective of this paper is to present a new method to quantify the level of signal distortion in TCSPC FLIM experiments, in order to determine the most efficient laser repetition rate for different FLT ranges. Optimization of the F -value, which is the relation between the relative standard deviation (RSD) in the measured FLT to the RSD in the measured fluorescence intensity (FI), allows quantification of the level of FI signal distortion, as well as determination of the correct FLT of the measurement. It is shown that by using a very high repetition rate (80 MHz) for samples characterized by high real FLT's (4-5 ns), virtual short FLT components are added to the FLT histogram while a F -value that is higher than 1 is obtained."
According to the news editors, the research concluded: "For samples characterized with short real FLT's, virtual long FLT components are added to the FLT histogram with the lower repetition rate (20-50 MHz), while by using a higher repetition rate (80 MHz) the 'inter-pulse pile-up' is eliminated as the F -value is close to 1."
For more information on this research see: The influence of dead time related distortions on live cell fluorescence lifetime imaging (FLIM) experiments. Journal of Biophotonics, 2014;7(6):442-52. (Wiley-Blackwell - www.wiley.com/; Journal of Biophotonics - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648)
The news editors report that additional information may be obtained by contacting L. Turgeman, Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, 52900, Israel (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Israel, Ramat Gan.
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