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Investigators from Huazhong Agricultural University Target Vibrio parahaemolyticus (Specific Detection of Vibrio Parahaemolyticus by Fluorescence...

August 19, 2014



Investigators from Huazhong Agricultural University Target Vibrio parahaemolyticus (Specific Detection of Vibrio Parahaemolyticus by Fluorescence Quenching Immunoassay Based on Quantum Dots)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Proteobacteria have been published. According to news reporting originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "In this study, anti-Vibrio parahaemolyticus polyclonal and monoclonal antibodies were prepared through intradermal injection immune and lymphocyte hybridoma technique respectively. CdTe quantum dots (QDs) were synthesized at pH 9.3, 98 A degrees C for 1 h with stabilizer of 2.7:1."

Our news editors obtained a quote from the research from Huazhong Agricultural University, "The fluorescence intensity was 586.499, and the yield was 62.43 %. QD probes were successfully prepared under the optimized conditions of pH 7.4, 37 A degrees C for 1 h, 250 mu L of 50 mg/mL EDC A center dot HCl, 150 mu L of 4 mg/mL NHS, buffer system of Na2HPO4-citric acid, and 8 mu L of 2.48 mg/mL polyclonal antibodies. As gold nanoparticles could quench fluorescence of quantum dots, the concentration of V. parahaemolyticus could be detected through measuring the reduction of fluorescence intensity in immune sandwich reaction composed of quantum dot probe, gold-labeled antibody, and the sample. For pure culture, fluorescence intensity of the system was proportional with logarithm concentration of antigen, and the correlation coefficient was 99.764 %. The fluorescence quenching immunoassay based on quantum dots is established for the first time to detect Vibrio parahaemolyticus."

According to the news editors, the research concluded: "This method may be used as rapid testing procedure due to its high simplicity and sensitivity."

For more information on this research see: Specific Detection of Vibrio Parahaemolyticus by Fluorescence Quenching Immunoassay Based on Quantum Dots. Applied Biochemistry and Biotechnology, 2014;173(5):1073-1082. Applied Biochemistry and Biotechnology can be contacted at: Humana Press Inc, 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. (Springer - www.springer.com; Applied Biochemistry and Biotechnology - www.springerlink.com/content/0273-2289/)

The news editors report that additional information may be obtained by contacting L. Wang, Huazhong Agricultural University, Coll Food Sci & Technol, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, People's Republic of China. Additional authors for this research include J.X. Zhang, H.L. Bai, X. Li, P.T. Lv and A.L. Guo (see also Proteobacteria).

Keywords for this news article include: Asia, Wuhan, Quantum Dots, Vibrionaceae, Nanotechnology, Quantum Physics, Gammaproteobacteria, Emerging Technologies, Gram-Negative Bacteria, Vibrio parahaemolyticus, People's Republic of China, Gram-Negative Facultatively Anaerobic Rods

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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