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Investigators at University of Pennsylvania Have Reported New Data on Transport Vesicles (Local cytoskeletal and organelle interactions impact...

August 19, 2014

Investigators at University of Pennsylvania Have Reported New Data on Transport Vesicles (Local cytoskeletal and organelle interactions impact molecular-motor- driven early endosomal trafficking)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Transport Vesicles have been published. According to news reporting from Philadelphia, Pennsylvania, by NewsRx journalists, research stated, "In the intracellular environment, motor-driven cargo must navigate a dense cytoskeletal network among abundant organelles. We investigated the effects of the crowded intracellular environment on early endosomal trafficking."

The news correspondents obtained a quote from the research from the University of Pennsylvania, "Live-cell imaging of an endosomal cargo (endocytosed epidermal growth factor-conjugated quantum dots) combined with high-resolution tracking was used to analyze the heterogeneous motion of individual endosomes. The motile population of endosomes moved toward the perinuclear region in directed bursts of microtubule-based, dynein-dependent transport interrupted by longer periods of diffusive motion. Actin network density did not affect motile endosomes during directed runs or diffusive interruptions. Simultaneous two-color imaging was used to correlate changes in endosomal movement with potential obstacles to directed runs. Termination of directed runs spatially correlated with microtubule-dense regions, encounters with other endosomes, and interactions with the endoplasmic reticulum. During a subset of run terminations, we also observed merging and splitting of endosomes, deformation of the endoplasmic reticulum, and directional reversals at speeds up to 10-fold greater than characteristic in vitro motor velocities. These observations suggest that endosomal membrane tension is high during directed run termination. Our results indicate that the crowded cellular environment significantly impacts the motor-driven motility of organelles."

According to the news reporters, the research concluded: "Rather than simply acting as impediments to movement, interactions of trafficking cargos with intracellular obstacles may facilitate communication between membrane-bound compartments or contribute to the generation of membrane tension necessary for fusion and fission of endosomal membranes or remodeling of the endoplasmic reticulum."

For more information on this research see: Local cytoskeletal and organelle interactions impact molecular-motor- driven early endosomal trafficking. Current Biology, 2013;23(13):1173-80. (Elsevier -; Current Biology -

Our news journalists report that additional information may be obtained by contacting A.L. Zajac, The Pennsylvania Muscle Institute and Dept. of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6085, United States. Additional authors for this research include Y.E. Goldman, E.L. Holzbaur and E.M Ostap (see also Transport Vesicles).

Keywords for this news article include: Endosomes, Organelles, Philadelphia, Pennsylvania, United States, Nanotechnology, Molecular Motors, Transport Vesicles, Cellular Structures, Intracellular Space, Emerging Technologies, Endoplasmic Reticulum, Cytoplasmic Structures, North and Central America.

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Source: Life Science Weekly

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