Findings from University of Michigan in the Area of Nanorods Reported (Plasmon-Enhanced Brightness and Photostability from Single Fluorescent Proteins Coupled to Gold Nanorods)
By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Nanorods is the subject of a report. According to news reporting out of Ann Arbor, Michigan, by NewsRx editors, research stated, "Single-molecule imaging pushes fluorescence microscopy beyond the diffraction limit of traditional microscopy. Such super-resolution imaging, which relies on the detection of bright, stable fluorescent probes to achieve nanometer-scale resolution, is often hindered in biological systems by dim, blinking fluorescent proteins (FPs)."
Our news journalists obtained a quote from the research from the University of Michigan, "Here, we use gold nanorods and single-molecule fluorescence detection to achieve plasmon-enhanced emission from intrinsically fluorescent proteins. We measure a doubled photon emission rate from the red FP mCherry and detect three times more photons before photobleaching from the photoactivatable FP PAmCherry. We further explore the effect of near-field nanorod interactions on the yellow FP mCitrine, for which the observed emission enhancements cannot overcome measurable quenching. Overall, our work indicates that plasmonic particles improve both the brightness and photostability of FPs and extends the applications of plasmon-enhanced fluorescence to the arena of biological imaging."
According to the news editors, the research concluded: "Furthermore, because gold nanorods are nontoxic, they are promising extracellular imaging substrates for enhancing emission from FP-labeled membrane-bound proteins in live cells."
For more information on this research see: Plasmon-Enhanced Brightness and Photostability from Single Fluorescent Proteins Coupled to Gold Nanorods. Journal of Physical Chemistry C, 2014;118(27):15027-15035. Journal of Physical Chemistry C can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry C - www.pubs.acs.org/journal/jpccck)
Our news journalists report that additional information may be obtained by contacting J.E. Donehue, University of Michigan, Dept. of Chem, Ann Arbor, MI 48109, United States. Additional authors for this research include E. Wertz, C.N. Talicska and J.S. Biteen (see also Nanorods).
Keywords for this news article include: Michigan, Ann Arbor, United States, Nanotechnology, Emerging Technologies, North and Central America
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