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Findings from University of Adelaide in the Area of Electrophoresis Reported (High resolution two-dimensional electrophoresis of native proteins)

August 19, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Electrophoresis have been published. According to news reporting out of Adelaide, Australia, by NewsRx editors, research stated, "Blue native PAGE (BN-PAGE) is a powerful method to separate protein complexes while preserving their native state. However, the resolution of the method is limited as complexes with similar molecular masses cannot be resolved."

Our news journalists obtained a quote from the research from the University of Adelaide, "Here we describe native 2DE using immobilized pH-gradients in combination with BN-PAGE to resolve protein complexes by their pI and molecular mass. This method enables electrophoretic separation of proteins between pI 3 and 10 and can resolve molecular masses up to 1.2 MDa. Visualized gel spots at large molecular weight were identified using MS to confirm potential protein complexes. Several protein complexes could be identified, most prominent GroEL in complex with GroES, parts of the ribosomal machinery and membrane transport system."

According to the news editors, the research concluded: "In summary, this method enables easy high-resolution electrophoretic separation of protein complexes."

For more information on this research see: High resolution two-dimensional electrophoresis of native proteins. Electrophoresis, 2014;35(12-13):1893-1902. Electrophoresis can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell -; Electrophoresis -

Our news journalists report that additional information may be obtained by contacting F. Weiland, University of Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia. Additional authors for this research include C.M. Zammit, F. Reith and P. Hoffmann (see also Electrophoresis).

Keywords for this news article include: Adelaide, Electrophoresis, Australia and New Zealand

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Source: Life Science Weekly

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