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Data on Microbiology Reported by Researchers at Natural History Museum (Diverse molecular signatures for ribosomally 'active' Perkinsea in marine...

August 19, 2014



Data on Microbiology Reported by Researchers at Natural History Museum (Diverse molecular signatures for ribosomally 'active' Perkinsea in marine sediments)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Life Science Research. According to news reporting from London, United Kingdom, by NewsRx journalists, research stated, "Perkinsea are a parasitic lineage within the eukaryotic superphylum Alveolata. Recent studies making use of environmental small sub-unit ribosomal RNA gene (SSU rDNA) sequencing methodologies have detected a significant diversity and abundance of Perkinsea-like phylotypes in freshwater environments."

The news correspondents obtained a quote from the research from Natural History Museum, "In contrast only a few Perkinsea environmental sequences have been retrieved from marine samples and only two groups of Perkinsea have been cultured and morphologically described and these are parasites of marine molluscs or marine protists. These two marine groups form separate and distantly related phylogenetic clusters, composed of closely related lineages on SSU rDNA trees. Here, we test the hypothesis that Perkinsea are a hitherto under-sampled group in marine environments. Using 454 diversity 'tag' sequencing we investigate the diversity and distribution of these protists in marine sediments and water column samples taken from the Deep Chlorophyll Maximum (DCM) and sub-surface using both DNA and RNA as the source template and sampling four European offshore locations. We detected the presence of 265 sequences branching with known Perkinsea, the majority of them recovered from marine sediments. Moreover, 27% of these sequences were sampled from RNA derived cDNA libraries. Phylogenetic analyses classify a large proportion of these sequences into 38 cluster groups (including 30 novel marine cluster groups), which share less than 97% sequence similarity suggesting this diversity encompasses a range of biologically and ecologically distinct organisms. These results demonstrate that the Perkinsea lineage is considerably more diverse than previously detected in marine environments. This wide diversity of Perkinsea-like protists is largely retrieved in marine sediment with a significant proportion detected in RNA derived libraries suggesting this diversity represents ribosomally 'active' and intact cells."

According to the news reporters, the research concluded: "Given the phylogenetic range of hosts infected by known Perkinsea parasites, these data suggest that Perkinsea either play a significant but hitherto unrecognized role as parasites in marine sediments and/or members of this group are present in the marine sediment possibly as part of the 'seed bank' microbial community."

For more information on this research see: Diverse molecular signatures for ribosomally 'active' Perkinsea in marine sediments. Bmc Microbiology, 2014;14():110. (BioMed Central - www.biomedcentral.com/; Bmc Microbiology - www.biomedcentral.com/bmcmicrobiol/)

Our news journalists report that additional information may be obtained by contacting A. Chambouvet, Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK. Additional authors for this research include C. Berney, S. Romac, S. Audic, F. Maguire, C. De Vargas and T.A Richards (see also Life Science Research).

Keywords for this news article include: London, Europe, United Kingdom, Life Science Research.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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