Data on Cancer Gene Therapy Reported by Researchers at Shanxi Medical University (Construction of plasmid vector pAFP-HSVtk-IRES2-EGFP and its effect on the cytotoxicity of ganciclovir to hepatocellular carcinoma)
By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators publish new report on Biotechnology. According to news reporting from Taiyuan, People's Republic of China, by NewsRx journalists, research stated, "Herpes simplex virus thymidine kinase phosphorylates ganciclovir to ganciclovir monophosphate, which is then converted to ganciclovir triphosphate by endogenous cellular nucleoside kinases. The ganciclovir triphosphate acts as a DNA chain terminator due to the lack of a functional 3'-OH group and terminates the process of DNA replication, hence leading to cell apoptosis."
The news correspondents obtained a quote from the research from Shanxi Medical University, "At present, HSVtk gene usually acts as suicide gene to kill tumor cells. The aim of this study was to investigate the selective cytotoxicity of the herpes simplex virus thymidine kinase/ganciclovir (HSVtK/GCV) suicide gene system controlled by the a-fetoprotein (AFP) promoter on hepatocellular carcinoma (HCC) cells in vitro. Methods pAFP-HSVtk-IRES2-EGFP recombinant plasmid vectors driven by the AFP promoter were constructed. HL-7702 liver cells, HUH-7 HCC, and HepG2 HCC were transfected with the recombinant plasmids. HSVtK gene expression was detected using Western blotting analysis. HepG2 cells line stably expressing HSVtk gene was selected by G418 reagent. The cytotoxicity of HSVtK/GCV suicide gene system on hepatoma cells was measured by CCK-8 reagents when different doses of ganciclovir were added. Plasmid pAFP-TK-IRES2-EGFP-expressed HSVtk gene was constructed successfully. HSVtk gene expression level was significantly higher in AFP-positive hepatoma cells than in AFP-negative liver cells. After G418 selection, a HepG2 cells line stably expressing HSVtk gene was acquired. With the increase of the dose of ganciclovir the optical density at 450 nm of HepG2 cells stably expressing HSVtk gene gradually decreased (P
According to the news reporters, the research concluded: "The HSVtK gene-specific expression in hepatoma cells as well as the cytotoxicity of the suicide gene system in HepG2 cells provided the basis for the targeted gene therapy of HCC."
For more information on this research see: Construction of plasmid vector pAFP-HSVtk-IRES2-EGFP and its effect on the cytotoxicity of ganciclovir to hepatocellular carcinoma. Chinese Medical Journal, 2014;127(12):2337-2341. Chinese Medical Journal can be contacted at: Chinese Medical Assoc, 42 Dongsi Xidajie, Beijing 100710, Peoples R China. (Chinese Medical Association - www.cmj.org; Chinese Medical Journal - www.ecmj.org.cn/ch/first_menu.aspx?parent_id=20070904111927001)
Our news journalists report that additional information may be obtained by contacting Z.Y. Lai, Shanxi Med Univ, Dept. of Biochem & Mol Biol, Taiyuan 030001, Shanxi, People's Republic of China. Additional authors for this research include Q. Qin, B.F. Yu, J. Xie, R.P. Gao, T.T. Zhang, C.F. Li, K. Niu and J. Xu (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Taiyuan, Oncology, Proteins, Hepatomas, Viral DNA, Proteomics, Gastroenterology, Thymidine Kinase, Cancer Gene Therapy, Enzymes and Coenzymes, Hepatocellular Carcinoma, People's Republic of China
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