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Data from Punjabi University Provide New Insights into Enzymes and Coenzymes [Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb...

August 20, 2014



Data from Punjabi University Provide New Insights into Enzymes and Coenzymes [Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk]

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Enzymes and Coenzymes have been published. According to news reporting out of Patiala, India, by NewsRx editors, research stated, "The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples."

Our news journalists obtained a quote from the research from Punjabi University, "Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6 mu m was achieved in the milk and the enzyme membranes were stable for more than two months at 4 degrees C. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 mu m. Lead specificity was attained by means of masking agents 1,10 - phenanthroline and sodium potassium tartarate."

According to the news editors, the research concluded: "Validation of the developed biosensors was carried out with spiked milk samples."

For more information on this research see: Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk. Brazilian Archives of Biology and Technology, 2014;57(4):613-619. Brazilian Archives of Biology and Technology can be contacted at: Inst Tecnologia Parana, Rua Prof Algacyr Munhoz Mader 3775-Cic, 81350-010 Curitiba-Parana, Brazil (see also Enzymes and Coenzymes).

Our news journalists report that additional information may be obtained by contacting H. Kaur, Punjabi Univ, Biosensor Technol Lab, Dept. of Biotechnol, Patiala, Punjab, India. Additional authors for this research include S. Kumar and N. Verma.

Keywords for this news article include: Asia, India, Patiala, Biosensing, Bioengineering, Bionanotechnology, Nanobiotechnology, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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